Robust and Systematic Drug Screening Method Using Chemical Arrays and the Protein Library : Identification of Novel Inhibitors of Carbonic Anhydrase II
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概要
- 論文の詳細を見る
The identification of specific interactions between small molecules and human proteins of interest is a fundamental step in chemical biology and drug development. Here we describe an efficient method to obtain novel binding ligands of human proteins by a chemical array approach. Our method includes large-scale ligand screening with two libraries, proteins and chemicals, the use of cell lysates that express proteins of interest fused with red fluorescent protein, and high-throughput screening by merged display analysis, which removes false positive signals from array experiments. Using our systematic platform, we detected novel inhibitors of carbonic anhydrase II. It is suggested that our systematic platform is a rapid and robust approach to screen novel ligands for human proteins of interest.
- 社団法人 日本農芸化学会の論文
- 2008-10-23
著者
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Kawatani Makoto
Antibiotics Lab. And Chemical Biology Dep. Riken
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Osada Hiroyuki
Antibiotics Laboratory Riken Discovery Research Institute
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Osada Hiroyuki
Antibiotic Laboratory Riken (institute Of Physical And Chemical Research)
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Osada Hiroyuki
Antibiotics Laboratory And Chemical Biology Department Riken
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Miyazaki Isao
Antibiotics Laboratory And Chemical Biology Department Riken
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Simizu Siro
Antibiotics Laboratory And Chemical Biology Department Riken
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ICHIMIYA Harumi
Antibiotics Laboratory and Chemical Biology Department, RIKEN
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Ichimiya Harumi
Antibiotics Laboratory And Chemical Biology Department Riken
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Simizu Siro
Antibiotics Laboratory And Chemical Biology Department Advanced Science Institute Riken
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