Specific regulation of cytokine-dependent p38 MAP kinase activation by p62/SQSTM1
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概要
- 論文の詳細を見る
We have previously shown that p62/SQSTM1 binds to p38. In this study, we identified two association domains of p62 to p38 by conducting co-immunoprecipitation experiments. One domain comprises the amino acids 173182, named N-terminal p38 interaction (NPI) domain, and the other domain comprises the amino acids 335344, named C-terminal p38 interaction (CPI) domain. An aspartic acid tripeptide located at 335337 was required for their association. However, the direct interaction was only observed between the recombinant p38 and the peptide of the NPI domain, but not that of the CPI domain in the surface plasmon resonance analyses. These results suggest that the CPI domain may serve to form a certain conformation suitable for the association with p38. Furthermore, we showed that knockdown of p62 expression by siRNA led to impaired p38 phosphorylation only when HeLa cells were stimulated by cytokine. The critical role of p62 in cytokine-dependent p38 signalling pathway was further confirmed by measuring IL-8 mRNA. Cytokine mRNA is often stabilized via p38 pathway. In the absence of p62, IL-8 mRNA induced by IL-1 became more fragile. These data show that p62 specifically regulates cytokine-dependent p38 signalling pathway.
- 社団法人日本生化学会の論文
- 2008-06-01
著者
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OSADA Hiroyuki
Antibiotics Laboratory, Discovery Research institute, RIKEN
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Saito Akiko
Antibiotics Laboratory And Bioarchitect Research Group Dri Riken
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Osada Hiroyuki
Antibiotics Laboratory And Bioarchitect Research Group Dri Riken
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KAWAI Kayoko
Antibiotics Laboratory and Bioarchitect Research Group, DRI, RIKEN
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SUDO Tatsuhiko
Antibiotics Laboratory and Bioarchitect Research Group, DRI, RIKEN
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Osada Hiroyuki
Antibiotic Laboratory Riken (institute Of Physical And Chemical Research)
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Kawai Kayoko
Antibiotics Laboratory And Bioarchitect Research Group Dri Riken
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Sudo Tatsuhiko
Antibiotics Laboratory And Bioarchitect Research Group Dri Riken
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