Disposition of protein-bound 3-nitrotyrosine in rat plasma analysed by a novel protocol for HPLC-ECD
スポンサーリンク
概要
- 論文の詳細を見る
3-Nitrotyrosine (NTyr) is considered as a biomarker of the generation of reactive nitrogen species (RNS). However, it is still difficult to determine its concentration in biological samples. To develop a reliable and high-throughput method, we optimized the conditions for high performance liquid chromatography and electrochemical detection (HPLC-ECD). The best separation of NTyr was achieved using a highly acidic mobile phase (pH 2.5). The concentration of protein-bound NTyr in plasma protein was 593.6 ± 53.8 fmol/mg in rats treated with lipopolysaccharide (LPS) and 114.4 ± 27.6 fmol/mg in control. After intravenous administration of in vitro-nitrated plasma protein, NTyr concentration decreased; the half-life was 63.4 ± 16.8 h. Consistently, protein-bound NTyr concentration in plasma after LPS treatment declined gradually, but was detectable for 1 week. Our protocol is reproducible and suitable for analysing multiple clinical samples to study RNS production in vivo. © 2007 The Japanese Biochemical Society.
- 日本生化学会 = Japanese Biochemical Societyの論文
- 2007-04-01
著者
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OGINO Keiki
Department of Public Health, Okayama University Graduate School of Medicine, Dentistry & Pharmaceuti
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KAMBAYASHI Yasuhiro
Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa Un
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OHNO Hideki
Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine
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HIBINO Yuri
Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa Un
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Taniguchi Toshiyo
Dep. Of Welfare System And Health Sci. Okayama Prefectural Univ.
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Hibino Yuri
岡山大学 医歯薬学総合研究科公衆衛生学
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Hibino Yuri
Department Of Environmental And Preventive Medicine Graduate School Of Medical Science Kanazawa Univ
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Ogino Keiki
中国四国安全衛生サービスセンター
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Ogino Keiki
Department Of Public Health Okayama University Graduate School Of Medicine Dentistry And Pharmaceuti
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Ogino K
Department Of Public Health Okayama University Graduate School Of Medicine Dentistry & Pharmaceu
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Ogino K
Medical School Kanazawa University
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Ogino Keiki
Dep. Of Public Health Okayama Univ. Graduate School Of Medicine Dentistry And Pharmaceutical Science
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TANIGUCHI Naoyuki
Department of Biochemistry Osaka University Medical School
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Hitomi Yoshiaki
Department Of Molecular Predictive Medicine And Sport Science Kyorin University School Of Medicine
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Hitomi Yoshiaki
金沢大学 医学系研究科環境社会医学
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Hitomi Yoshiaki
Department Of Environmental And Preventive Medicine Graduate School Of Medical Science Kanazawa Univ
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Takigawa Tomoko
Department Of Public Health Okayama University Graduate School Of Medicine Dentistry And Pharmaceuti
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Ogino Keiki
Department Of Public Health Okayama University Graduate School Of Medicine Dentistry & Pharmaceu
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Ogino Keiki
Department Of Environmental And Preventive Medicine Graduate School Of Medical Science Kanazawa Univ
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NISHINO Hirohito
Eicom Ltd.
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Ohno Hideki
Department Of Hygiene National Defense Medical College
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Taniguchi Naoyuki
Department Of Biochemistry Osaka University Graduate School Of Medicine
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Takigawa Tomoko
Department Of Public Health Okayama University Graduate School Of Medical Dentistry And Pharmaceutic
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OKUDA Junna
Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa Un
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TAKEMOTO Kei
Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceu
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Okuda Junna
Department Of Environmental And Preventive Medicine Graduate School Of Medical Science Kanazawa Univ
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Takemoto Kei
Department Of Public Health Okayama University Graduate School Of Medicine Dentistry And Pharmaceuti
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Kameda Yukie
Department Of Environmental And Preventive Medicine Graduate School Of Medical Science Kanazawa Univ
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Kameda Yukie
Department Of Health Sciences Graduate School Of Medical Science Kanazawa University
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Takigawa Tomoko
Department Of Welfare System And Health Science Okayama Prefectural University
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Taniguchi Naoyuki
Department Of Disease Glycomics Research Institute For Microbial Diseases Osaka University
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Kambayashi Yasuhiro
Department Of Environmental And Preventive Medicine Graduate School Of Medical Science Kanazawa Univ
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Kambayashi Yasuhiro
Department Of Photon And Free Radical Research Japan Immunoresearch Laboratories
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Hibino Yuri
Department Of Environmental And Preventive Medicine Graduate School Of Medical Science Kanazawa Univ
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Ohno Hideki
Department Of Gastroenterology Graduate School Of Medicine University Of Tokyo
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Ohno Hideki
Department Of Molecular Predictive Medicine And Sport Science Kyorin University School Of Medicine
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Nishino Hirohito
Eicom Corporation
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Nishino Hirohito
Eicom Co. Ltd.
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Hibino Yuri
Department Of Environmental And Preventive Medicine Faculty Of Medicine Institute Of Medical Pharmaceutical And Health Sciences Kanazawa University
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Kambayashi Yasuhiro
Department Of Environmental And Preventive Medicine Faculty Of Medicine Institute Of Medical Pharmaceutical And Health Sciences Kanazawa University
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Hitomi Yoshiaki
Department Of Environmental And Preventive Medicine Faculty Of Medicine Institute Of Medical Pharmaceutical And Health Sciences Kanazawa University
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OHNO Hideki
Department of Gastroenterology and Cancer Gene Regulation, Kanazawa University Graduate School of Medical Sciences
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