REESTIMATION OF CYPRIDINA LUCIFERIN ANALOGS (MCLA) AS A CHEMILUMINESCENCE PROBE TO DETECT ACTIVE OXYGEN SPECIES : CAUTIONARY NOTE FOR USE OF MCEA
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概要
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Cypridina luciferin analogs have been widely used as a specific chemiluminescence probe for the detection of superoxide anion (O_2^-) and singlet oxygen (1^O_2). However, light emission during the reaction of Cypridina luciferin analogs and other active oxygen species (AOS) has not been reported in detail. Therefore, we re-evaluated 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazine-3-one (MCLA), one of the Cypridina luciferin analogs, as a chemiluminescence probe to detect various AOS. MCLA-dependent chemiluminescence was observed when MCLA was incubated with the following systems; 1) hypoxanthine plus xanthine oxidase (O_2^-), 2) thermolysis of endoperoxide (1^O_2), 3) hydrogen peroxide plus ferrous ion (hydroxyl radical), 4) ferrous ion, 5) thermolysis of azo compound (alkyl peroxyl radical) and 6) hydrogen peroxide. Superoxide dismutase inhibited MCLA-dependent chemiluminescence observed during ferrous ion-induced decomposition of hydrogen peroxide. Alkyl peroxyl radical reacted with MCLA, but light was not emitted when the concentration of MCLA was high. These results suggest that radicals, except O_2^-, appeared not to be direct inducers of MCLA-dependent light emission. In summary, MCLA-dependent chemiluminescence was induced by various AOS in addition to O_2^- and ^1O_2, but active species must be O_2^- and 1^O_2 in many cases. These points should be appreciated when Cypridina luciferin analogs, such as MCLA, are used for the detection of AOS.
- 日本トキシコロジー学会の論文
- 2003-08-29
著者
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OGINO Keiki
Department of Public Health, Okayama University Graduate School of Medicine, Dentistry & Pharmaceuti
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KAMBAYASHI Yasuhiro
Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa Un
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Ogino Keiki
中国四国安全衛生サービスセンター
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Ogino Keiki
Department Of Public Health Okayama University Graduate School Of Medicine Dentistry And Pharmaceuti
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Ogino K
Department Of Public Health Okayama University Graduate School Of Medicine Dentistry & Pharmaceu
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Ogino K
Medical School Kanazawa University
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Ogino Keiki
Department Of Public Health Okayama University Graduate School Of Medicine Dentistry & Pharmaceu
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Ogino Keiki
Department Of Environmental And Preventive Medicine Graduate School Of Medical Science Kanazawa Univ
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Kambayashi Y
Japan Immunores. Lab. Takasaki
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Kambayashi Yasuhiro
Department Of Environmental And Preventive Medicine Graduate School Of Medical Science Kanazawa Univ
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Kambayashi Yasuhiro
Department Of Environmental And Preventive Medicine Faculty Of Medicine Institute Of Medical Pharmaceutical And Health Sciences Kanazawa University
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