Micropatterning Oligonucleotides on Single-Crystal Diamond Surface by Photolithography
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概要
- 論文の詳細を見る
DNA micropatterns have been for the first time fabricated on a single-crystal diamond surface in conjunction with the photolithography technique. A new chemical modification process for producing amine groups inside patterned regions and a passivation layer terminated with fluorine outside patterned regions is demonstrated. The resulting amine groups within patterned areas and fluorine termination outside patterned areas on the single-crystal diamond surface were characterized by spatially resolved X-ray photoelectron spectroscopy. Amine-terminated oligonucleotides were then linked to the amine-patterned regions using a crosslinker. It was revealed that hybridization on DNA-patterned diamond is specific and selective, with a low background outside the patterns and strong binding to complementary probe DNA immobilized inside the patterns but no binding to noncomplementary probe DNA similarly immobilized inside the patterns. These results suggest that DNA micropatterning on a single-crystal diamond may serve as an ideal platform for future biochips and biosensors.
- Japan Society of Applied Physicsの論文
- 2005-02-10
著者
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Funatsu Takashi
Laboratory Of Bio-analytical Chemistry Graduate School Of Pharmaceutical Sciences The University Of
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Zhang Guo-jun
Nanotechnology Research Center Waseda University
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Hata Hideo
Department Of Electronical Engineering And Bioscience School Of Science And Engineering Waseda Unive
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Zako Tamotsu
Laboratory Of Bio-analytical Chemistry Graduate School Of Pharmaceutical Sciences The University Of
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Umezawa Hitoshi
Department Of Electronical Engineering And Bioscience School Of Science And Engineering Waseda Unive
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Ohdomari Iwao
Nanotechnology Research Center Waseda University
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Kawarada Hiroshi
Nanotechnology Research Center Waseda University
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Funatsu Takashi
Laboratory of Bio-Analytical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
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Zako Tamotsu
Laboratory of Bio-Analytical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
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Umezawa Hitoshi
Department of Electronical Engineering and Bioscience, School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
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Hata Hideo
Department of Electronical Engineering and Bioscience, School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
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Kawarada Hiroshi
Nanotechnology Research Center, Waseda University, 513 Waseda Tsurumaki-cho, Shinjuku-ku, Tokyo 162-0041, Japan
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