Vascular Endothelial Growth Factor Principally Acts as the Main Angiogenic Factor in the Early Stage of Human Osteoblastogenesis
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概要
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Vascular endothelial growth factor (VEGF)-mediated angiogenesis is essential for bone formation. However, the effect of VEGF on osteoblastic cells during osteoblast-ogenesis is still controversial. The aim of this study was to clarify the relationship between osteoblastic cells derived from human mesenchymal stem cells (MSCs) and VEGF in the early stage of osteoblastic differentiation. Continuous dexamethasone treatment with a low concentration stimulated osteoblastogenesis of MSCs and the expression of VEGF121 mRNA. The VEGF secretion from osteoblastic cells also increased along with osteoblastogenesis. Neuropilin-1, which mainly binds VEGF165, was detected at all stages during early osteoblastogenesis, but VEGF receptor-1 and -2 were not detected on RT PCR analyses. In this study, VEGF had no direct effect on the proliferation of osteoblastic cells. However, the secreted VEGF in the conditioned medium of osteoblastic cells exhibited high angiogenic power as to endothelial cell proliferation. Our findings indicated that VEGF121 principally acts as the main ang-iogenic factor in the early stage of human osteoblastogenesis. The present study also demonstrated the differential expression of VEGF121 during osteoblastogenesis. The increase of VEGF in the early stage might be a useful marker of induction of bone for-mation due to human MSCs.
- 社団法人 日本生化学会の論文
著者
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Ninomiya Yoshifumi
Department Of Molecular Biology And Biocemistry Okayama University Medical School
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Oohashi Toshitaka
Department Of Molecular Biology And Biochemistry Okayama University Graduate School Of Medicine And
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Nishida Keiichiro
Department Of Human Morphology Okayama University Graduate School Of Medicine Dentistry And Pharmace
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Furumatsu Takayuki
Department Of Molecular Biology And Biochemistry Okayama University Graduate School Of Medicine And
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MANABE Hironori
Department of Molecular Biology and Biochemistry Okayama University Graduate School of Medicine and
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Kawai Akira
Department Of Electrical Engineering Nagaoka University Of Technology
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Inoue Hajime
Department Of Applied Chemistry Faculty Of Engineering Kanagawa University
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Shen Zheng
Department Of Child Health Care The Children's Hospital Of Zhejiang University School Of Medici
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