Hypoxia Increases Transforming Growth Factor-β1 Concomitantly with Types I and III Collagen without Further Enhancement by Reoxygenation in Cultured Rat Cardiac Fibroblasts
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概要
- 論文の詳細を見る
The effects of oxygen concentration and reoxygenation following hypoxic culture on transforming growth factor-β1(TGF-β1)expression and types I and III collagen[α2(I)and α1(III)]mRNA expression were examined in neonatal rat cardiac fibroblasts cultured with medium containing 10% or 1% fetal calf serum at confluency. The oxygen tension was varied using a N_2-CO_2-O_2 controllable incubator. Cells were exposed to continuous hypoxia(2% O_2)or were reoxygenated(21% O_2)after hypoxic culture. The TGF-β1 protein expression level was determined by an enzyme-linked immunosorbent assay, and the expression levels of TGF-β1, α2(I)and α1(III)mRNA were measured by a Northern blot analysis. Both the TGF-β1 mRNA and protein levels were higher in the continuous hypoxic cultures than in the continuous normoxic cultures. Their levels in reoxygenation cultures were not substantially different from those in the continuous normoxic cultures. Similarly, the expressions of both α2(I)and α1(III)mRNA were enhanced in continuous hypoxic culture, and no differences were obtained between normoxic and reoxygenation cultures. The present results indicate that hypoxia induces high expression levels of TGF-β1 and types I and III collagen in rat cardiac fibroblasts and reoxygenation does not further enhance this hypoxia-induced upregulation.
- 日本結合組織学会の論文
- 1998-09-25
著者
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Tsuji T
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine And Dentistry
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二宮 善文
岡山大学大学院 医歯薬学総合研究科 分子医化学
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二宮 善文
岡山大学 第2解剖
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NINOMIYA Yoshifumi
Department of Molecular Biology and Biochemistry, Okayama University Medical School
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DOI Masayuki
Department of Internal Medicine, Division of Cardiology, Sumitomo Besshi Hospital
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Tsuji Takao
First Department Of Internal Medicine Okayama University School Of Medicine
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Kusachi Shozo
First Department Of Internal Medicine Okayama University Medical School
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Murakami Masaaki
Cardiovascular Medicine The Sakakibara Heart Institute Of Okayama
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Kondo Jun
First Department Of Internal Medicine Okayama University Medical School
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NUNOYAMA Hiroshi
First Department of Internal Medicine
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OOHASHI Toshitaka
Department of Molecular Biology and Biochemistry, Okayama University Medical School
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MURAKAMI Masahiro
First Department of Internal Medicine
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MURAKAMI Takashi
First Department of Internal Medicine
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OHNISHI Hiromichi
First Department of Internal Medicine
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DOI Masayuki
First Department of Internal Medicine
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Ninomiya Yoshifumi
Department Of Molecular Biology And Biocemistry Okayama University Medical School
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Okigaki T
Division Of Social Welfare Faculty Of Social Welfare Kinki Welfare University
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Doi Masayuki
Department Of Internal Medicine Division Of Cardiology Sumitomo Besshi Hospital
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Nunoyama Hiroshi
First Department Of Internal Medicine Okayama University Medical School
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Oohashi Toshitaka
Departments Of Molecular Biology And Biochemistry Okayama University Graduate School Of Medicine And
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Oohashi Toshitaka
Department Of Molecular Biology And Biochemistry Okayama University Medical School
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Oohashi Toshitaka
Department Of Molecular Biology And Biochemistry Okayama University Graduate School Of Medicine Dent
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Oohashi Toshitaka
Department Of Molecular Biology And Biochemistry Okayama University Graduate School Of Medicine And
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Ohnishi Hiromichi
Department Of Cardiology Kagawa Prefectural Central Hospital
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Tsuji Takao
The First Depertment Of Internal Medicine Okayama University Medical School
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Doi Masayuki
Departments Of Cardiology Iwakuni National Hospital
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Ninomiya Yoshifumi
Department Of Molecular Biology And Biochemistry Okayama University Graduate School Of Medicine Dent
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Oohashi T
Department Of Molecular Biology And Biochemistry Okayama University Graduate School Of Medicine Dent
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Tsuji Takao
First Department Of Internal Medicine Okayama University Medical School
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Taguchi Takehito
Department Of Radiological Technology Faculty Of Health Sciences Okayama University School Of Medici
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Murakami Masaaki
Department Of Cardiovascular Medicine Okayama University Graduate School Of Medicine Dentistry And P
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KONDO JUN
First Department of Internal Medicine, Okayama University Medical School
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