Distribution of Basement Membrane Type IV Collagen Molecules in Choroid Plexus, Pia Mater and Capillaries in Murine Brain

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We investigated differential distribution of basement membrane type IV collagen α chains in murine brain by immunohistochemistry using α chain-specific monoclonal antibodies. Subendothelial basement membranes contained cd and α2 chains. Basement memb-anes surrounding smooth muscle cells on vessel walls were stained for cd and α2 chains but not for α5 and α6 chains. Interestingly, pia mater contained a thin basement membrane, which was positive for α1, α2, α5, and α6 chains, suggesting that glia limitans superficialis coheres basement membranes containing [α1(IV)]_2α2(IV) and [α5(IV)]_2α6(IV) molecules. In contrast, capillaries always possessed thin basement membranes of [α1(IV)]_2α2(IV) molecules. Cerebrospinal fluid is produced through filtration of blood at choroid plexus, where two distinct basement membranes were detected by anti-cd and anti-α2 antibodies. Subendothelial basement membrane appeared to consist of [α1(IV)]_2α2(IV) molecules, whereas subependymal basement membrane in choroid plexus was strongly positive for α3, α4, and α5 chains, indicating that the filtering unit was composed of α3(IV)α4(IV)α5(IV) molecules. Considering the specific locations of these molecules such as glomerulus and choroid plexus, we hypothesize that supramolecular network containing α3(IV)α4(IV)α5(IV) molecules may functon as a permeability selective barrier. (Supported in part by Grants-in-Aid from the Japan Society for the Promotion of Science.)
日本結合組織学会の論文

Distribution of Basement Membrane Type IV Collagen Molecules in Choroid Plexus, Pia Mater and Capillaries in Murine Brain

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