Expression of .BETA.3-Adrenoceptor and Stimulation of Glucose Transport by .BETA.3-Agonists in Brown Adipocyte Primary Culture.

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概要

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• Precursor cells of brown adipocytes were isolated from the interscapular brown fat of newborn rats and cultured on collagen-coated dishes. When confluent cells were treated with dexamethasone, mRNAs for muscle/adipocyte type of glucose transporter, hormonesensitive lipase, and CCAAT/enhancer binding protein α were increased remarkably, confirming a predominant effect of dexamethasone on the terminal differentiation of the cultured cells. Effects of dexamethasone on the expression of three subtypes of β-adrenoceptor were also examined., β1- and β2-adrenoceptor mRNAs remained constant regardless of dexamethasone-treatment, while β3-adrenoceptor mRNA was present only in dexamethasone-treated differentiated cells. To assess the metabolic response mediated by β3-adrenoceptor, glucose transport into the cells was estimated. Norepinephrine enhanced glucose transport in dexamethasone-treated differentiated cells, but not in undifferentiated cells. β3-Adrenergic agonists mimicked completely the stimulatory effect of norepinephrine at concentrations lower by two orders of magnitude. These results suggest that the β3-adrenoceptor is expressed during the course of differentiation in brown adipocytes and plays a significant role in the response of glucose transport to adrenergic stimulation.

• 社団法人 日本生化学会の論文

著者

• Shimazu Takashi

  Department Of Cardiology Osaka University Graduate School Of Medicine

• Nikami Hideki

  Laboratory Of Animal Experiment Institute Of Immunological Science Hokkaido University

• Yoshida Toshihide

  First Departmen T Of Internal Medicine Kyoto Prefectural University Of Medicine

• Sumida Michihiro

  Department Of Biochemistry Ehime University School Of Medicine

• Minokoshi Yasuhiko

  Deparment Of Medical Biochemistry Ehime University School Of Medicine

• Shimizu Yasutake

  Department Of Medical Biochemistry Ehime University School Of Medicine

• Saito Masayuki

  Laboratories Of Biochemistry Department Of Biomedical Sciences Hokkaido University Graduate School O

• Nikami Hideki

  Laboratory of Biochemistry, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University

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