Purification and Structure of Novel Cysteine Proteinase Inhibitors, Staccopins P1 and P2, from Staphylococcus tanabeensis(Biological Chemistry)

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概要

• 論文の詳細を見る

• A new type of cysteine proteinase inhibitors, staccopin P1 and P2, are low molecular weight (lower than 1,000) peptide derivatives isolated first from Staphylococcus sp. Staccopins strongly inhibited only cysteine proteinases like calpain and papain, but were not active against serine proteinases like trypsin and chymotrypsin. The purification procedures included HP-20 adsorption chromatography, DEAE-cellulose, CM-cellulose, Sephadex LH-20 column chromatography, and high-performance liquid chromatography. The yields were 49 mg and 18 mg from 20 liters each of culture fluid. These proteinase inhibitors are pentapeptides containing valine and phenylalaninal for staccopin P1 or tyrosinal for staccopin P2, and the N-terminals of both peptides are free. Thus the structures are H-valyl-valyl-valyl-valyl-phenylalaninal for staccopin P1 and H-valyl-valyl-valyl-valyl-tyrosinal for staccopin P2. The amino acid sequence-inhibitory activity relationships of staccopins and other low molecular weight peptide cysteine proteinase inhibitors derived from natural sources are discussed.

• 社団法人日本農芸化学会の論文
• 1987-03-22

著者

• NOMOTO Kyosuke

  Suntory Institute for Bioorganic Research

• Nomoto Kyosuke

  Faculty of Life Science, Toyo University

• Nomoto Kyosuke

  Faculty Of Life Science Toyo University

• Nomoto Kyosuke

  Suntory Institute Of Bioorganic Research

• Nomoto Kyosuke

  Suntory Institute For Bioorganic Reseach

• Saito Masayuki

  Laboratory of Biochemistry, Department of Biomedical Sciences, Graduate School of Veterinary Medicin

• Nomoto Kyosuke

  Faculty Of Life Sciences Toyo University

• Kodama Tohru

  Laboratory Of Biochemistry Laboratories Of Applied Microbiology Suntory Research Center

• Tanaka T

  Tohoku Univ. Sendai Jpn

• Nomoto K

  Faculty Of Life Science Toyo University

• KAWAGUCHI Naoko

  Laboratory of Biochemistry, Laboratories of Applied Microbiology, Suntory Research Center

• HASHIMOTO Masaki

  Laboratory of Biochemistry, Laboratories of Applied Microbiology, Suntory Research Center

• HIGUCHI Naoki

  Laboratory of Biochemistry, Laboratories of Applied Microbiology, Suntory Research Center

• TANAKA Takaharu

  Laboratory of Biochemistry, Laboratories of Applied Microbiology, Suntory Research Center

• MURACHI Takashi

  Department of Clinical Science, Kyoto University Faculty of Medicine

• Higuchi Naoki

  Suntory Research Center

• Saito M

  Laboratory Of Biochemistry Laboratories Of Applied Microbiology Suntory Research Center

• Hashimoto Masaki

  Laboratory Of Biochemistry Laboratories Of Applied Microbiology Suntory Research Center

• Murachi T

  Department Of Clinical Science Kyoto University Faculty Of Medicine

• Murachi Takashi

  Department Of Clinical Science Faculty Of Medicine Kyoto University

• Kawaguchi Naoko

  Laboratory Of Biochemistry Laboratories Of Applied Microbiology Suntory Research Center

• Saito Masayuki

  Laboratories Of Biochemistry Department Of Biomedical Sciences Hokkaido University Graduate School O

• TANAKA Takaharu

  Laboratory of Applied Microbiology, Research Center

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