Immunohistochemical Lacalization of Annetocin, an Earthworm Oxytocin-Related Peptide, and Identification and Ultrastructural Characteristics of the Annetocin-Secretory Cells in the Oligochaete Earthworm Eisenia foetida

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概要

• 論文の詳細を見る

• Annetocin is an egg-laying-inducing oxytocin-related peptide which we have previously isolated from the earthworm, Eisenia foetida. Here we report the results of immunohistochamical and ultrastructural studies on annetocin-secretory cells in the earthworm. Annetocin-immunoreactive(IR)cell-somata were located mainly at the ventro-lateral side of the subesophageal ganglion. Only four annetocin-IR cells were seen in the cerebral ganglion. Some annetocin-IR cells displayed unipolar-like structure with a process directing to the core region(the neuropile)of the ganglion. Annetocin-IR fibers were also observed in the neuropile of the ventral ganglia and the ventral nerve cord between the 4th and the 30th segments including the clitellum, but not in the posterior segments(31-55th). The number of annetocin-IR fibers decreased from the 4th to the 30th segment. The annetocin-secretory cells were identified by the immunogold staining, and filled with gold-labeled vesicles, 200-250 nm in diameter, which included moderately electron dense material. The annetocin-secretory cells possessed a euchromatic nucleus, well-developed rough endoplasmic reticulum and Golgi apparatus. Some of the annetocin-secretory cells were found to form a neurohemal-like structure, where somata or fibers with loose glial investiment came in contact with the coelomic space at the ventral side of the subesophageal ganglion. The results suggest that annetocin is a neuropeptide produced and secreted by the neuron in the cerebral and subesophageal ganglia of the earthworm.

• 社団法人日本動物学会の論文

著者

• MINAKATA Hiroyuki

  Suntory Institute for Bioorganic Research

• Takahama Hideki

  Department Of Biology School Of Dental Medicine Tsurumi University

• Takahama Hideki

  Department Of Biology Faculty Of Education Oita University

• 南方 宏之

  (財)サントリー生有研

• Ukena K

  Department Of Biological Science Faculty Of Science Hiroshima University

• Takahama H

  Department Of Biology Faculty Of Education Oita University

• Furukawa Y

  Faculty Of Integrated Arts And Sciences Hiroshima University

• NOMOTO Kyosuke

  Suntory Institute for Bioorganic Research

• Matsushima Osamu

  Department of Environmental Information Faculty of Environmental Study Hiroshima Institute of Techno

• Nomoto K

  Suntory Institute For Bioorganic Research

• Nomoto Kyosuke

  Suntory Institute For Bioorganic Reseach

• Furukawa Yasuo

  Department Of Biological Science Graduate School Of Science Hiroshima University

• MORISHITA Fumihiro

  Department of Biological Science, Graduate School of Science, Hiroshima University

• 南方 宏之

  財団法人サントリー生物有機科学研究所

• OUMI Tomoyuki

  Department of Biological Science, Faculty of Science, Hiroshima University

• UKENA Kazuyoshi

  Department of Biological Science, Faculty of Science, Hiroshima University

• Haibara Kumi

  Department of Biology, Faculty of Education, Oita University

• Matsushima Osamu

  Fuculty Of Environmental Studies Hiroshima Institute Of Technology

• Haibara Kumi

  Department Of Biology Faculty Of Education Oita University

• Oumi Tomoyuki

  Department Of Biological Science Faculty Of Science Hiroshima University

• Morishita F

  Department Of Biological Science Hiroshima University

• Morishita Fumihiro

  Department Of Biological Science Graduate School Of Science Hiroshima University

• Minakata Hiroyuki

  Suntory Foundation For Life Sciences

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