Studies on the Fatty Acid Specificities of Acyl-CoA Synthetase

概要

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We studied on the substrate specificities of various fatty acids for acyl-CoA synthetase in the determination of serum free fatty acids, with use of acyl-CoA synthetase, myokinase, pyruvate kinase and lactate dehydrogenase as an assay system. The formation of acyl-CoA with acyl-CoA Synthetase from free fatty acids in serum and from purified fatty acids of Ci16: 0, C18: 0, C18: 1 and C18: 2 reached almost the maximum within 10 min incubation at 37°C. On the contrary, it needed to reach the maximum for more than 190 min in C12: 0, 80 min in C22: 6, 65 min in C20: 5, 45 min in C18: 3, 35 min in C20: 2 and 22 min in C14: 0. Significantly different apparent Km values were obtained for C12: 0 (0.3×10-2M), C20: 5 (0.5×10-3M) and C18: 1 (0.9×10-4M). These results suggest that there are consistent substrate specificities for acyl-CoA synthetase among various kinds of fatty acids available in human serum.
Japan Society of Clinical Chemistryの論文