骨芽細胞に対するメナキノン-4の作用

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It has been reported that vitamin K plays an important role in bone formation in vivo. But the mechanism is still veiled. We investigated the effect of an active form menaquinone-4 (MK-4, 2-methyl-3-all-trans-tetraprenyl-1, 4-naphtoquinone ; menatetrenone) on human osteoblastic function. Human osteoblasts derived from ulnar periosteum which show various characteristic features for osteoblasts, were cultured in α-MEM containing 10% fetal bovine serum. When confluent cells were exposed to MK-4 (2.25×10^<-6>M) in the presence of 2mM α-glycerophosphate for 21 days, mineralization in the cells was enhanced by the treatment. MK-4-induced mineralization was facilitated by the addition of 10^<-8> or 10^<-9> M 1,25-dihydroxyvitamin D_3, in which the effect had been already seen on day 5 of the treatment. The accumulation of osteocalcin, the vitamin K-dependent bone Gla protein, on cell layer was enhanced by MK-4 treatment, while the osteocalcin released into medium was not enhanced by the treatment. On the other hand the production of prostaglangin (PG) E_2, one of bone resorbing agents, was inhibited by MK-4 treatment. Our data suggested that the mineralization in osteoblasts induced by MK-4 was probably caused by the enhancement of osteocalcin accumulation on cell layer, and that MK-4 not only enhanced mineralization, but also might inhibit bone resorption by the inhibition of PGE_2 production. Both the enhancement of mineralization and the inhibition of PGE_2 production in human osteoblasts may contribute to the stimulative effects of MK-4 on bone formation in vivo.
日本ビタミン学会の論文
1993-06-25

骨芽細胞に対するメナキノン-4の作用

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