Isoketopinic Acid Loading Test for Conjugation and Excretion Ability

概要

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s an aid for designing a drug administration procedure, isoketopinic acid loading test was carried out to search the conjugation and excretion ability of patients. Isoketopinic acid, 300 /mole, was dissolved in 50 ml of water containing 50 mg of NaHCO3, and orally administered to fasting subjects, and 2 and 4 hours later, urines were collected. One ml of the urine was added with 03 mg of cinnamic acid as an internal standard, extracted twice with 4 ml of ether. The ether phase was dried with Na2SO4 and reacted with diazomethane. After evaporation of the solvent, the methylated sample was dissolved in 0.2 ml of acetone and analysed by gas chromatography to determine the content of free isoketopinic acid. The content of total isoketopinic acid was similarly determined after hydrolysis of the glucuronide by adding 03 ml of 1M NaOH to 1 ml of urine and heating at 70° for 10 min. The content of conjugate isoketopinic acid was obtained by subtracting the value of the free from that of the total. About 50% of administered isoketopinic acid was excreted in 2 hr in urine of normal subjects, about 70% in 4 hr, mainly as the conjugate (TABLE I). Larger deviation in excretion amounts of total and conjugate isoketopinic acid was observed with hepatitis patients (TABLE II, III), whileclear decrease was observed with 2/3 of liver cirrhosis patients (TABLE IV) and a half of SMON patients (TABLE VI) tested.
Japan Society of Clinical Chemistryの論文