A Rapid Determination Method for the Groups of Glycine-and Taurine-Conjugated Bile Acids in the Bile by HPLC

概要

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A rapid method for determining simultaneously the total concentration of group of glycine-(G) or taurine-(T) conjugated bile acids was developed using high-pressure liquid chromatography on a column of an anion exchange packing, TSK Gel IEX 540 DEAE (80×8mm i. d.).<BR>With 0.017 M sodium citrate buffer (pH 3.6)-EtOH (3: 2, v/v) as an eluent, the bile acids in each group were eluted at almost the same retention time. On this condition, G and T were per- fectly separated in less than 10 min.<BR>Each bile acid showed the same response to the differential refractometer. The values of each group obtained by this method were coincided with those by the fluorometry using 3α-hydroxysteroid: NAD oxidoreductase-NAD system. Good reproducibility was obtained and the added bile acids to the bile were recovered quantitatively.<BR>This method was applied to the human bile samples pretreated simply from normal subjects and from patients suffering from various hepatobiliary diseases.
Japan Society of Clinical Chemistryの論文