Application of High-Performance Liquid Chromatography (HPLC) with a Chemiluminescence Detection System to Determine Catecholamines in Urine and Plasma

概要

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Chemiluminescence generated with the reaction of bis (2, 4, 6,-trichlorophenyl) oxalate and hydrogen peroxide was applied to a detection system for HPLC to determine fluorescamine-labeled catecholamines. The sensitivity of the chemiluminescence detection system was 25fmol for fluorescamine-labeled dopamine. Norepinephrine and dopamine in human urine were determined by the use of the new HPLC detection system with the coefficient of variation of less than 4.0%. Good correlations were obtained between the values for both norepinephrine and dopamine by the present method and the convetional fluorescence method.<BR>In order to extend the utility of the new method we are tryin g to apply it to the determination of plasma catecholamines. However, for the determination of them in plasma, more sensitivity is required. For the purpose, we replaced the photomultiplier to a more sensitive one, changed the shape of a flow cell to a spiral type and re-investigated chemiluminescence reaction conditions from the view point of time courses of the reaction.
Japan Society of Clinical Chemistryの論文