Preparation of Specific Antibodies to Catecholamines and L-DOPA

概要

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A method for preparation of antigens of catecholamines and L-DOPA as haptens was developed. These haptens were conjugated with carrier proteins by the Mannich reaction after Nmaleylation of the haptens for protecting the amino groups of the side chain, then the maleyl groups were removed by acid hydrolysis under a mild condition. By immunization of rabbits with these conjugates antibodies were produced. In a cross-reactivity test by Ouchterlony's method the antibodies to each one of the conjugates selectively reacted with the conjugate of that hapten even if the carrier protein was bovine or rabbit serum albumin. The binding activity of the antibody to L-epinephrine was tested by Millipore filtration. The binding of 3 H-epinephrine was specifically inhibited by epinephrine but only slightly by the other catecholamines and their metabolites. The preliminary experiment of enzyme immunoassay for epinephrine was attempted using the antibody.
Japan Society of Clinical Chemistryの論文