「Salicylate培地」および「NH2OH培地」による非定型抗酸菌のScreening 土壌抗酸菌による不全型感染が示唆された2例について

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It is not difficult to detect scotochromogenic mycobacteria in clinical specimens by cultivations, but it is not easy to detect other atypical mycobacteria. It is desired to find a simple method for screening of the mycobacteria other than M. tuberculosis and M bovis. The senior author presented previously the salicylate medium and the hydroxylamine (NH<SUB>2</SUB>OH . HC1) medium as useful aids for differentiation of M. tuberculosis and M. bovis (Tsukamura, M.: Amer. Rev. Resp. Dis., 86: 81, 1962; Tsukamura, M.. J. Bacteriol., 90: 556, 1965). In the present study, we used these media for the detection of atypical mycobacteria in clinical specimens and obtained good results. The media were used incorporated in series of medium for the drug resistance tests. The mycobacteria other than tubercle bacilli (114. tuberculosis and M. bovis) were detected on these media as organisms showing growth similar to growth on control medium. The results are shown in Table 1. We could find five cases excr e ting atypical mycobacteria in their sputum (Mori was found in January 1967). Patient Mori and patient Usui excreted only once, patient Miyaji and patient Tsutsui four times and patient Yoshida six times. These strains isolated showed characters as shown in Table 2. Strains Miyaji, Usui and Mori were identified as M. terrae, strain Tsutsui as M. novum, and strain Yoshida as M. fortuitum subsp. runyonii. Since strain Yoshida was described previously (Tsukamura, M., Tsukamura, S. & Itasaka, Y.. Kekkaku, 42: 213, 1967), the present study deals with strains Miyaji and Tsutsui. Mode of excretion, form of disease and chemotherapy are shown in Table 3. These cases, Miyaji and Tsutsui, were suggested to have abortive infection with M. terrae or M. novum. The reason for this suggestion is as follows: (1) Aoki et al. (Aoki, M., Osato, & Kudo, S.: Jap. J. Chest Dis., 25: 814, 1966) suggested from their statistical observations that excretion of more than three times of atypical mycobacteria from the same patient is probably due to something more than simple contamination. Patients Miyaji and Tsutsui excreted four times M. terrae and M. novum, respectively; (2) These cases excreted only M. terrae or M. novum, tubercle bacilli being not observed after January 1966; (3) The cases showed a stronger reaction to nonphotochromogen Gamoh-n. than did to H<SUB>32</SUB>Rv-π (The result supports an infection with strain Miyaji, as M. terrae forms the M. a vium-group with M. intracellulare (pathogenic nonphotochromogen); (4) These cases showed a good general condition and had old non-cavitary lesions with bronchiektasia. Appendix. Among isola t es obtained in 1962, we found a strain (Inoue) of M. parafortuitunc. This organism is a soil Mycobacterium and has first been found in sputum. The characters of this strain are shown in Table 2. An identification table which w e use preliminarily for unknown mycobacteria is shown as an appendix table.
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「Salicylate培地」および「NH2OH培地」による非定型抗酸菌のScreening 土壌抗酸菌による不全型感染が示唆された2例について

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