Enumeration of Viable Tubercle Bacilli in Sputum by Spiral Loop Inoculation

概要

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Enumeration of viab le tubercle bacilli in sputum is thought to be important as basis of determination of viable tubercle bacilli excreted in sputa per day, determination of viable numbers of tubercle bacilli per ml of sputum, and of quantitative expression of content of drug-resistant bacilli, which are useful for clinical purposes. Recently, Ogawa and Ootani (Kekkaku, 37: 577-581, 1962) reported on the method of enumeration of viable tubercle bacilli in sputum, by which was obtained successful actual count at a rate of 78 per cent of the test cases. They employed three different dilutions of sputum fluid, such as 10-2, 10-3 and 10-4 (a continuous series), and pipette inoculation. The degree of dilution was determined according to the Gaffky number of smears stained by the Ziehl-Neelsen method. On the other hand, the present authors had reported an improved method of enumeration of viable numbers of tubercle bacilli occurring in sputa (Tsukamura, M.: Medicine & Biology, 49: 87-90, 1958; Tsukamura, M. et al.: Kekkaku, 34: 625-633, 1959). This method proved to be successful for determination of viable numbers at a rate as high as 98 per cent.
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