Purification and characterization of calciumbinding protein containing .GAMMA.-carboxyglutamic acid from rat bone.
スポンサーリンク
概要
- 論文の詳細を見る
A γ-carboxyglutamic acid (Gla)-containing protein was purified from rat femur cortical bone. The presence of 4 Gla residues in the rat protein was shown by amino acid analysis on alkaline and acid hydrolysates. The protein was extracted from rat cortical bone with 0.5 M EDTA (pH 7.6) and purified from the EDTA extracts by gel filtration on Sephadex G-100 and ion-exchange chromatography on DEAE-Sephadex A-25. The protein has a molecular weight of about 6, 000 on the basis of amino acid composition. The protein had 56 amino acid residues containing significant amounts of Asp, Glu, and Gla (acidic amino acids). The protein showed calcium-binding activity with Kd=0.2 mM and calcium-dependent electrophoretic mobility.
- 財団法人 学会誌刊行センターの論文
著者
-
奥山 典生
Department Of Chemistry Faculty Of Sciece Tokyo Metropolitan University:(present Address) Department
-
太田原 洋子
東京大学医学部保健栄養学教室
-
細谷 憲政
Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
-
森内 幸子
Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
-
太田原 洋子
Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
-
笠井 久隆
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University
-
森内 幸子
Department of Food and Nutrition, Faculty of Home Economics, Japan Women's University
-
奥山 典生
Department of Chemistry, Faculty of Science, Tokyo Metropolitan University
-
細谷 憲政
Department fo Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
関連論文
- THE MINI-COLUMN OF LANTHANUM TREATED HYDROXYAPATITE CERAMIC BEADS FOR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF PROTEIN
- CHROMATOGRAPHY OF PROTEIN ON HYDROXYAPATITE IMPREGNATED PAPER
- ビタミンK研究の現状と展望 : 日本ビタミン学会第33回大会ワークショップ(ビタミン研究の現状)
- Hydrophobic Chromatography of Tubulin on Immobilized Colchicine Columns with Various Spacers
- 4 Vitamin Dの光増感酸化反応による6,19-epidioxyvitamin D類の合成と反応および生理活性
- 免疫学的方法並びに高速液体クロマトグラフィーによるシロネズミ骨におけるビタミンK依存性カルシウム結合タンパク質の定量
- 1-II-19 骨のビタミンK依存性カルシウム結合タンパク質の加齢に伴う変動 : 第34回大会研究発表要旨 : 日本ビタミン学会
- 2. 加令に伴うカルシウム代謝並びに骨のK依存性カルシウム結合タンパク質の変動 : 第196回会議研究発表要旨 : 脂溶性ビタミン総合研究委員会
- "科学"の源流
- STUDY ON HPLC OF AROMATIC AMINO ACIDS AND OLIGO PEPTIDES USING MICROSPHERICAL CARBON PACKINGS : HPLC of Amino Acids and Oligo Peptides on Carbon Column
- 3. ニワトリ骨のk依存性タンパク質の性状に及ぼすDの影響 : 第196回会議研究発表要旨 : 脂溶性ビタミン総合研究委員会
- 1-II-24 シロネズミ大腿骨のビタミンK依存性カルシウム結合タンパク質の一次構造について : 日本ビタミン学会第33回大会研究発表要旨
- ニワトリ大腿骨のビタミンK依存性カルシウム結合タンパク質の性状に及ぼすビタミンDの影響
- ヒト血清中の骨γ-カルボキシグルタミン酸含有タンパク質の加齢による変動
- 骨のγ-カルボキシグルタミン酸含有タンパク質のラット骨並びに血清中含有量に及ぼすビタミンDの影響
- 骨とビタミンK
- 1-II-16 ニワトリの発達過程における骨のビタミンK依存性カルシウム結合タンパク質の変動 : 第35回大会一般研究発表要旨 : 日本ビタミン学会
- 1-II-15 骨のビタミンK依存性カルシウム結合タンパク質に及ぼすビタミンDの影響 : 第35回大会一般研究発表要旨 : 日本ビタミン学会
- 骨のビタミンK依存性タンパク質に関する最近の知見
- Effect of Aging and Castration on the Changes in the Levels of Bone γ-Carboxyglutamic Acid-Containing Protein in Bone and Serum of Female Rat
- γカルボキシグルタミン酸含有蛋白の構造と機能 (γカルボキシグルタミン酸含有蛋白)
- 骨のビタミンK依存性タンパク質と1,25-(OH)_2-D_3
- 骨のビタミンK依存性タンパク質の一次構造
- シロネズミ大腿骨に含まれるビタミンK依存性カルシウム結合タンパク質の性質並びにヒドロキシアパタイト結晶化に及ぼす影響 : 日本ビタミン学会第32回大会一般研究発表要旨
- 動脈硬化とビタミンK依存性CaBP
- 骨におけるγ-カルボキシグルタミン酸含有タンパク質の合成と機能
- 4.シロネズミ十二指腸におけるビタミンD依存性カルシウム輸送能のカドミウムによるin vitroの阻害(JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY : 掲載論文要旨)
- 1-I-6 カドミウムの腸管吸収に及ぼすビタミンDの影響(日本ビタミン学会 : 第30回大会一般研究発表要旨)
- シロネズミ十二指腸のカドミウム輸送に及ぼす食餌中のカルシウム含量並びにビタミンD_3の影響
- γ-カルボキシルグルタミン酸を含むタンパク質とビタミンK
- A New Aspect on the Mechanism of Intestinal Cholesterol Absorption in Rat
- EFFECT OF VARIOUS SUGARS ON THE INDUCTION OF CHICK EMBRYONIC INTESTINAL DISACCHARIDASES IN THE ORGAN CULTURE SYSTEM
- INDUCTION OF CHICK EMBRYONIC INTESTINAL DISACCHARIDASES BY HYDROCORTISONE AND SUCROSE IN THE ORGAN CULTURE SYSTEM
- THE EFFECT OF 1, 25-DIHYDROXYCHOLECALCIFEROL ON THE MULTIPLE FORMS OF ALKALINE PHOSPHATASE AND THE SIALIC ACID INCORPORATION INTO MICROSOMES OF CHICK DUODENUM
- Property and Physiological Role of Biliary Secretory IgA in Rats
- VITAMIN D3 EFFECT ON THE CALCIUM CONTENT IN SUBCELLULAR FRACTIONS OF RAT INTESTINAL MUCOSA
- INHIBITORY EFFECT OF CADMIUM ON VITAMIN D STIMULATED CALCIUM TRANSPORT IN RAT DUODENUM IN VITRO
- THE EFFECT OF VITAMIN D3 AND DIETARY CALCIUM LEVEL ON THE CADMIUM-INDUCED MORPHOLOGICAL AND BIOCHEMICAL CHANGES IN RAT INTESTINAL MUCOSA
- VISUALIZATION OF VITAMIN D-DEPENDENT CALCIUM BINDING PROTEIN IN CHICK INTESTINAL TISSUE BY IMMUNO-SCANNING ELECTRON MICROSCOPY
- Effect of Sucrose and Acarbose Feeding on the Development of Streptozotocin-Induced Diabetes in the Rat
- Biliary Immunoglobulins in Protein-Energy Malnourished Rats
- 1, 25-DIHYDROXYCHOLECALCIFEROL AND INDUCTION OF ALKALINE PHOSPHATASE IN ORGAN CULTURE
- Calcium-Binding Proteins in Bovine Milk: Calcium-Binding Properties and Amino Acid Composition
- Calmodulin-Like Fanction of Calcium-Binding Protein in Bovine Milk
- CHANGES OF IMMUNOREACTIVE SUCRASE-ISOMALTASE COMPLEX IN RAT SMALL INTESTINE DURING POSTNATAL DEVELOPMENT AND MATURATION ALONG THE VILLUS-CRYPT AXIS
- EFFECT OF FOOD RESTRICTION ON INTESTINAL DISACCHARIDASES IN STREPTOZOTOCIN-INDUCED DIABETES OF RAT
- SOME CHARACTERISTICS OF EARLY APPEARING ISOMALTASE IN INTESTINAL MUCOSA OF SUCKLING RAT
- DEVELOPMENTAL CHANGES IN THE SUCRASE-ISOMALTASE COMPLEX IN RAT INTESTINAL MUCOSA
- EFFECT OF EXOGENOUS POLYUNSATURATED FATTY ACIDS ON LIPOGENIC ENZYME ACTIVITY IN RAT LIVER UNDER FIXED CARBOHYDRATE CONSUMPTION
- Nitrogen Balance and Hepatic Gluconeogenesis in Rats Fed on Diets Containing Various Proportions of Carbohydrate and Fat
- CHARACTERIZATION OF STEROL CARRIER PROTEIN BINDING WITH 7-DEHYDROCHOLESTEROL AND VITAMIN D3
- BINDING OF 7-DEHYDROCHOLESTEROL TO STEROL CARRIER PROTEIN AND VITAMIN D3 EFFECT
- THE EFFECT OF 1α-HYDROXYVITAMIN D3 AND CORTISONE ON THE DEVELOPMENT OF CHICK DUODENAL ALKALINE PHOSPHATASE IN ORGAN CULTURE
- PROTEINS OF CHICK DUODENAL BRUSH BORDERS DURING DEVELOPMENTAL CHANGES
- Demonstration of Sucrase-Isomaltase Complex in Chick Intestine
- Effect of aging and castration on the changes in the levels of bone .GAMMA.-carboxyglutamic acid-containing protein in bone and serum of female rat.
- Purification and characterization of calciumbinding protein containing .GAMMA.-carboxyglutamic acid from rat bone.
- 1.ビタミンK依存性のカルシウム結合タンパク質について : シロネズミ大腿骨のγ-カルボキシグルタミン酸含有タンパク質(脂溶性ビタミン総合研究委員会 : 第181回会議研究発表要旨)
- シロネズミ大腿骨のビタミンK依存性カルシウム結合タンパク質 : ウシのオステオカルシンとの比較 : 日本ビタミン学会第31回大会研究発表要旨
- Biological activity of the endoperoxides derived from vitamin D derivatives by dye-sensitized photo-oxidation.
- BINDING PROTEINS FOR 1, 25-DIHYDROXYCHOLE-CARCIFEROL AND 25-HYDROXY-CHOLECARCIFEROL
- Competence of 1.ALPHA.,25-dihydroxyvitamin D3 receptor system in embryonic chick intestine.
- Interaction of 1.ALPHA.,24-dihydroxyvitamin D3 with the chick intestinal 1.ALPHA.,25-dihydroxyvitamin D3 receptor system.
- Intestinal absorption of dinitrophenyl-lysine and effect of immunization with dinitrophenylated bovine serum albumin.
- Possible role of insulin status in the increased lipogenic enzyme activity by dietary medium-chain triglyceride in rat liver.
- Changes in glucose uptake by and phlorizin binding to brush-border membrane vesicles of small intestine from streptozotocin-induced diabetic rats.
- The effect of 1,25-dihydroxycholecalciferol on the changes of bone .GAMMA.-carboxyglutamic acid-containing protein in bone and serum of developing chick.
- Digestion and absorption of sugars and sugar substitutes in rat small intestine.
- Effect of pyridoxal 5'-phosphate on the 1,25-dihydroxyvitamin D3 receptor system.
- Changes of sugar-evoked transmural potential differences in intestine of rats with streptozotocin-induced diabetes.
- Some properties of luminal sucrase and sucrase-isomaltase complex in rat small intestine.
- Some characteristics of cytosol binding protein for 1.ALPHA.,25-dihydroxycholecalciferol and 25-hydroxycholecalciferol in rat intestinal mucosa.
- Inhibition of .BETA.-sitosterol on intestinal cholesterol absorption in rat using in vivo dual isotope ratio method.
- Regulatory effect of calcium-regulating hormones on the synthesis and/or secretion of bone .GAMMA.-carboxyglutamic acid-containing protein in chick embryonic calvaria in vitro.
- Effect of dietary vitamin D3 and cadmium on the lipid composition of rat intestinal brush border membranes.
- Hypertrophic effect of unavailable carbohydrate on cecum and colon in rats.
- Bone .GAMMA.-carboxyglutamic acid-containing protein (BGP)-induced changes in chick embryonic calvaria in vitro.
- The changes of bone .GAMMA.-carboxyglutamic acid-containing protein in bone and serum of developing chick.
- Changes of the activity and content of sucrase-isomaltase complex in the intestinal mucosa during the development of streptozotocin-induced diabetes in rats.
- Effect of dietary medium chain triglyceride on lipogenic enzyme activity in rat liver.