Some properties of luminal sucrase and sucrase-isomaltase complex in rat small intestine.
スポンサーリンク
概要
- 論文の詳細を見る
Some properties of luminal sucrase4somaltase complex and the effect of luminal fluid on their complex were studied in rat small intestine. Luminal contents were collected by flushing the small intestine with the buffered solution. The enzyme activity was observed in luminal contents and intestinal mucosa. Sucrase and Isomaltase activities were located mainly in the intestinal mucosa. However, approximately 20% of sucrase and 10% of isomaltase activities of total small intestine were found in the luminal contents. A significant amount of sucrase without isomaltase activity, the molecular weight of which was estimated at about 140, 000 daltons, was found in the luminal supernatant of the distal intestine in addition to the complexed form of sucrase and isomaltase. The luminal sucrase and sucrase-isomaltase complex had similar properties such as Km values, optimal pH, molecular weights and antigenicity against anti sucrase-isomaltase antibody compared with brush border membrane-bound sucrase-isomaltase complex. Furthermore, the supernatant of the luminal contents of the ileum had a degradative effect on the isomaltase moiety of the purified sucrase-isomaltase complex and a free sucrase without isomaltase also appeared in vitro as observed in vivo. These results suggest that the sucrase-isomaltase complex is released into the intestinal lumen from the brush border membrane and that a luminal factor affects the degradation step of this enzyme as well as the biosynthesis of su-crase-isomaltase complex.
- 財団法人 学会誌刊行センターの論文
著者
-
森内 幸子
Department of Food and Nutrition, Faculty of Home Economics, Japan Women's University
-
細谷 憲政
Department fo Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
-
山田 和彦
Department fo Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
-
山田 和彦
Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo.
-
安部 真佐子
Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo.
-
細谷 憲政
Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo.
関連論文
- A New Aspect on the Mechanism of Intestinal Cholesterol Absorption in Rat
- EFFECT OF VARIOUS SUGARS ON THE INDUCTION OF CHICK EMBRYONIC INTESTINAL DISACCHARIDASES IN THE ORGAN CULTURE SYSTEM
- INDUCTION OF CHICK EMBRYONIC INTESTINAL DISACCHARIDASES BY HYDROCORTISONE AND SUCROSE IN THE ORGAN CULTURE SYSTEM
- THE EFFECT OF 1, 25-DIHYDROXYCHOLECALCIFEROL ON THE MULTIPLE FORMS OF ALKALINE PHOSPHATASE AND THE SIALIC ACID INCORPORATION INTO MICROSOMES OF CHICK DUODENUM
- Property and Physiological Role of Biliary Secretory IgA in Rats
- VITAMIN D3 EFFECT ON THE CALCIUM CONTENT IN SUBCELLULAR FRACTIONS OF RAT INTESTINAL MUCOSA
- INHIBITORY EFFECT OF CADMIUM ON VITAMIN D STIMULATED CALCIUM TRANSPORT IN RAT DUODENUM IN VITRO
- THE EFFECT OF VITAMIN D3 AND DIETARY CALCIUM LEVEL ON THE CADMIUM-INDUCED MORPHOLOGICAL AND BIOCHEMICAL CHANGES IN RAT INTESTINAL MUCOSA
- VISUALIZATION OF VITAMIN D-DEPENDENT CALCIUM BINDING PROTEIN IN CHICK INTESTINAL TISSUE BY IMMUNO-SCANNING ELECTRON MICROSCOPY
- Effect of Sucrose and Acarbose Feeding on the Development of Streptozotocin-Induced Diabetes in the Rat
- Biliary Immunoglobulins in Protein-Energy Malnourished Rats
- 1, 25-DIHYDROXYCHOLECALCIFEROL AND INDUCTION OF ALKALINE PHOSPHATASE IN ORGAN CULTURE
- Calcium-Binding Proteins in Bovine Milk: Calcium-Binding Properties and Amino Acid Composition
- Calmodulin-Like Fanction of Calcium-Binding Protein in Bovine Milk
- CHANGES OF IMMUNOREACTIVE SUCRASE-ISOMALTASE COMPLEX IN RAT SMALL INTESTINE DURING POSTNATAL DEVELOPMENT AND MATURATION ALONG THE VILLUS-CRYPT AXIS
- EFFECT OF FOOD RESTRICTION ON INTESTINAL DISACCHARIDASES IN STREPTOZOTOCIN-INDUCED DIABETES OF RAT
- SOME CHARACTERISTICS OF EARLY APPEARING ISOMALTASE IN INTESTINAL MUCOSA OF SUCKLING RAT
- DEVELOPMENTAL CHANGES IN THE SUCRASE-ISOMALTASE COMPLEX IN RAT INTESTINAL MUCOSA
- EFFECT OF EXOGENOUS POLYUNSATURATED FATTY ACIDS ON LIPOGENIC ENZYME ACTIVITY IN RAT LIVER UNDER FIXED CARBOHYDRATE CONSUMPTION
- Nitrogen Balance and Hepatic Gluconeogenesis in Rats Fed on Diets Containing Various Proportions of Carbohydrate and Fat
- CHARACTERIZATION OF STEROL CARRIER PROTEIN BINDING WITH 7-DEHYDROCHOLESTEROL AND VITAMIN D3
- BINDING OF 7-DEHYDROCHOLESTEROL TO STEROL CARRIER PROTEIN AND VITAMIN D3 EFFECT
- THE EFFECT OF 1α-HYDROXYVITAMIN D3 AND CORTISONE ON THE DEVELOPMENT OF CHICK DUODENAL ALKALINE PHOSPHATASE IN ORGAN CULTURE
- PROTEINS OF CHICK DUODENAL BRUSH BORDERS DURING DEVELOPMENTAL CHANGES
- Demonstration of Sucrase-Isomaltase Complex in Chick Intestine
- Effect of aging and castration on the changes in the levels of bone .GAMMA.-carboxyglutamic acid-containing protein in bone and serum of female rat.
- Purification and characterization of calciumbinding protein containing .GAMMA.-carboxyglutamic acid from rat bone.
- Biological activity of the endoperoxides derived from vitamin D derivatives by dye-sensitized photo-oxidation.
- BINDING PROTEINS FOR 1, 25-DIHYDROXYCHOLE-CARCIFEROL AND 25-HYDROXY-CHOLECARCIFEROL
- Competence of 1.ALPHA.,25-dihydroxyvitamin D3 receptor system in embryonic chick intestine.
- Interaction of 1.ALPHA.,24-dihydroxyvitamin D3 with the chick intestinal 1.ALPHA.,25-dihydroxyvitamin D3 receptor system.
- Intestinal absorption of dinitrophenyl-lysine and effect of immunization with dinitrophenylated bovine serum albumin.
- Possible role of insulin status in the increased lipogenic enzyme activity by dietary medium-chain triglyceride in rat liver.
- Changes in glucose uptake by and phlorizin binding to brush-border membrane vesicles of small intestine from streptozotocin-induced diabetic rats.
- The effect of 1,25-dihydroxycholecalciferol on the changes of bone .GAMMA.-carboxyglutamic acid-containing protein in bone and serum of developing chick.
- Digestion and absorption of sugars and sugar substitutes in rat small intestine.
- Effect of pyridoxal 5'-phosphate on the 1,25-dihydroxyvitamin D3 receptor system.
- Changes of sugar-evoked transmural potential differences in intestine of rats with streptozotocin-induced diabetes.
- Some properties of luminal sucrase and sucrase-isomaltase complex in rat small intestine.
- Some characteristics of cytosol binding protein for 1.ALPHA.,25-dihydroxycholecalciferol and 25-hydroxycholecalciferol in rat intestinal mucosa.
- Inhibition of .BETA.-sitosterol on intestinal cholesterol absorption in rat using in vivo dual isotope ratio method.
- Regulatory effect of calcium-regulating hormones on the synthesis and/or secretion of bone .GAMMA.-carboxyglutamic acid-containing protein in chick embryonic calvaria in vitro.
- Effect of dietary vitamin D3 and cadmium on the lipid composition of rat intestinal brush border membranes.
- Hypertrophic effect of unavailable carbohydrate on cecum and colon in rats.
- Bone .GAMMA.-carboxyglutamic acid-containing protein (BGP)-induced changes in chick embryonic calvaria in vitro.
- The changes of bone .GAMMA.-carboxyglutamic acid-containing protein in bone and serum of developing chick.
- Changes of the activity and content of sucrase-isomaltase complex in the intestinal mucosa during the development of streptozotocin-induced diabetes in rats.
- Effect of dietary medium chain triglyceride on lipogenic enzyme activity in rat liver.