ハムスター口腔癌形成過程における免疫組織化単的研究 : 第1報頬嚢粘膜癌におけるケラチンの局在について

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The distribution of intracellular keratin was studied in dysplasia and squamous cell carcinomas of the cheek pouch mucosa of the hamster induced by the application of a 0.5% DMBA mineral oil. The hamster cheek pouchs were excised, and placed in a cold aqueous solution of 1% acetic acid for 1 hour while stirring. The pouches were rinsed and placed in a shallow pan of distilled water. The epithelium was then gently freed from the underlying connective tissue with the use of wooden chopsticks. With this technique the epithelium was easily separated from the connective tissue. anti-keratin antibody was induced in rabbits against total keratin extracts from the squamous epithelium of the cheek pouch mucosa. The serum was tested by the indirect immunoperoxidase method (PAP method). Chilled acetonefixed, paraffin-embedded tissues were employed.<BR>Anti-keratin antibody against total keratin was directed toward cytoplasmic antigen of keratinocytes of spinous and granular layers of the hamster cheek pouch epithelium. No labeling could be detected in the basal cell layer. In the dysplastic lesions and squamous cell carcinomas the expression of keratin antigens was markedly diminished or not detected.<BR>The present study suggests that the difference of the staining intensities of keratin among the normal epithelium, dysplasia and squamous cell carcinoma is a valuable indicator of the malignant transformation in the cheek pouch mucosa.