Mucoraceaeの数属におけるキトサンの分布および性質

概要

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Two strains of Actinomucor, 6 strains of Circinella, 2 strains of Phycomyces, 32 strains of Rhizopus, and 4 strains of Zygorhynchus, all from the family Mucoraceae, were cultivated for 44 hr at 24°C on a rotatory shaker in a medium containing glucose, peptone, yeast extract, and inorganic salts. The harvested cells of each strain were treated with hot 2% sodium hydroxide to isolate the alkali-insoluble materials. The extraction of chitosan from the alkali-insoluble materials was carried out with 2% acetic acid at room temperature. The degrees of deacetylation of almost all chitosan preparations were in a range of 80 to 90%. The viscosities of 0.1% solution (in 0.5% acetic acid) of the preparations varied widely. Rhizopus strains exhibited relatively high yields of chitosan; the majority were in a range from 30 to 100mg per flask (containing 200ml of medium). The results from the present study were compared with previously reported studies on Absidia and Mucor strains.
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