交流通電したリン酸緩衝液によるEscherichia coli B休止菌体の発育阻害

概要

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Growth of resting Escherichia coli B cells was inhibited after pre-incubation of the cells in a 8/15 M phosphate buffer, pH 7.0,anaerobically exposed to an alternating current (AC) of 50 Hz at a current density of 600 mA/cm^2 and 34±3℃. The growth inhibition of the cells pre-incubated in the AC-exposed buffer could be evaluated by the method of growth delay analysis, and the integrated viability (ν) of the cells decreased with increasing time or temperature during pre-incubation. The decrease in ν of the cells was suppressed when cells were pre-incubated in the exposed buffer after the treatment of the buffer with adsorbents, such as activated charcoal powder or an anion exchange resin, or with heat at 121℃. Addition of cysteine in the exposed buffer had a protective effect on the decrease in ν of the cells.Ion chromatography and UV spectrophotometry revealed some interesting differences between the components of the buffer exposed and unexposed to AC : pyrophosphate, a kind of condensed phosphate, was detected in the exposed buffer but not in the unexposed buffer, and the concentration of orthophosphate decreased and that of hypophosphite increased with increasing AC-exposure time.
社団法人日本生物工学会の論文
1986-09-25