細菌による油脂の過酸化 : (第2報)大豆油分解細菌の分離とそれらにおけるリポキシゲナーゼの分布

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From 24 samples of garbage, soil, sewage and infected fats, 34 strains of bacteria, which were able to grow in a synthetic medium containing soybean oil as the sole source of carbon (Medium B on Table 1) were isolated (Table 3). Changes in peroxide value (POV) of soybean oil in media by these strains were measured during their incubation periods and compared with the POV change in the control, the medium without inoculum. And, lipoxygenase activities of filtrated media on the 10th incubation day were also measured by the modified Smith and Sumner's colorimetric method under reaction conditions of pH 10.9,37℃ for 10 minutes (Fig.1,Fig.2 and Table 4). From these data, all the strains were classified into the following 4 groups. A-4 type : 14 strains, in media of which increases in POV and enzyme activities were observed. A-12A type : 6 strains, in media of which decreases in POV, but enzyme activities were observed. A-1 type : 5 strains, in media of which increases in POV were observed, but enzyme activities were null or very small on both 10th and 20th days. A-11 type : 9 strains, in media of which POV changes were almost identical to that of control and no activity was observed. Because all of 20 strains classified into A-4 and A-12A types were identified as Pseudomonas aeruginosa as will be reported in the following paper, it is possible to postulate that enzymes formed by these strains were identical to that formed by A-4 strain of which some properties have been reported previously. When A-4 strain was incubated in medium B with a thinner soybean oil layer (Table 5), the POV change (Fig.3) appeared similar to that in usual medium B by A-12A strain, and, in both cases, since it was observed that pellicles were so developed to be exposed in the air, the culture conditions might be more aerobic than in the cases when increases in POV were observed. These facts may suggest that the same enzyme causes either the increase or the decrease in POV by a unclarified mechanism based on the oxygen supply into the medium. In the case of each strain in A-1 type, it is presumable that the substance(s) acting like lipoxygenase formed during its incubation. Probably an enzyme(s) having an optimal reaction condition in the lower range of pH than that from A-4 strain, etc., is the cause of the increase in PVO
公益社団法人日本生物工学会の論文
1966-05-25

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島原健三
成蹊大学工学部

細菌による油脂の過酸化 : (第2報)大豆油分解細菌の分離とそれらにおけるリポキシゲナーゼの分布

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