Pseudomonas aeruginosa A-4株を生産するLipoxygenase-like Enzymeの性質 : 細菌による油脂の過酸化(第7報)

概要

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A lipoxygenase-like enzyme preparation from Pseudomonas aeruginosa strain A-4 was purified about 25-fold usin ammonium sulfate salting, DEAE-cellulose column chromatography, and gel filtration with BioGel A-0.5m. Properties of the purified enzyme preparaiton were as follows : 1) The enzyme preparation aws stable below 60℃ and in a pH range from 2 to 11. 2) Optimal temperature for the reaction was between 37 and 40℃ and the optimal pH value was 10.8 and 11.0. 3) The activation energy of the reaction was 8.6 kcal/mole. 4) Methyl oleate and methyl linoleate were also found to be substrates of the enzyme preparation, but relative activities on these substrates compared with the standard substrate, methyl liinoleate, were 0.15 and 0.73 respectively. 5) Cyanide was an inhibitor of the enzyme reaction, but azide, fluoride, ethylenediaminetetracetic acid, p-chloromercuribenzoate, chloracetophenone, and monoiodoacetamide did not affect the reaction.
社団法人日本生物工学会の論文
1973-03-25