細菌による油脂の過酸化 : (第4報) Achromobacter delicatulus A-1株の生産する活性物質の性質

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In a previous paper, it was reported that peroxide was formed in soybean oil by Adelicatulus when incubated in a medium containing the oil as the sole source of carbon and that production of a substance acting like lipoxygenase was presumed. So, by using the filtrate of cultured strain A-1 as a crude active-substance solution, properties of this substance were studied manometrically. The reaciton conditions were usually at 37.5℃ and with pH 4.50 and crude linoleic acid shown in Table 1 as a substance.The substance was found to be stable in a wide range of pH between 4.5 to 12.2 and was not inactivated by both heat and protein-denaturating reagents. By distillation of the solution, about a half of the original activity was recovered in the distillate. The substance, therefore, might be a water-soluble one with a low molecular weight.The optimum pH for the reaction was about 4.5 (Fig. 3), but the reaction rate was increased as the temperature went up untill 75.5℃, that was the upper limit of the experiment (Fig. 4 and 5). α-Tocopherol behaved as an inhibitor (Fig. 6). Both C_<18> acids with conjugated dienes and their methyl esters proved to be good substrates, but C_<18> acids and their esters with unconjugated double bonds were far less effective than with conjugated dienes even if dienes had been interrupted by a methylene group.
社団法人日本生物工学会の論文
1968-01-25