Properties of Testosterone 5α-Reductase of Purified Nuclear Fraction from Ventral Prostate of Rats
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概要
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It was found that more than half of the activity of testosterone 5α-reduction was attached to nuclear fraction of rat ventral prostate, and some properties of this reaction were investigated using purified nuclear fraction of the gland.<BR>The optimum pH for reduction of testosterone was about 7.2 and required NADPH as a donor of reducing equivalent. Apparent km of nuclear 5α-reductase was 3.2×10<SUP>-5</SUP><I>M</I> for testosterone and 3.0×10<SUP>-4</SUP><I>M</I> for NADPH. Apparent Km for 19-nortestosterone was similar to that for testosterone, but the value for androst-4-ene-3, 17-dione was higher than that for testosterone.<BR>Natural and synthetic estrogenic compounds showed their inhibitory effect on the rate of testosterone 5α-reduction, but one of the gestagen and of anti-androgen did not inhibit the reaction. Estradiol-17β exhibited a most profound inhibition among the compounds examined, and the double reciprocal plots revealed that type of the inhibition is competitive.<BR>The reverse reaction of 5α-reductase using NADP or phenazine dimethyl sulfate as electron acceptor was not detected in purified nuclear fraction from rat ventral prostate.
- 社団法人 日本内分泌学会の論文
著者
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SHIDA KEIZO
Department of Urology, Gunma University School of Medicine
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Shimazaki Jun
Department Of Ophthalmology School Of Medicine Keio University
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OHKI YUMIKO
Department of Urology, Gunma University School of Medicine
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HORAGUCHI TATSUO
Department of Urology, Gunma University School of Medicine
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SHIMAZAKI JUN
Department of Dermatology, School of Medicine, Chiba University
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SHIDA KEIZO
Department of Urology School of Medicine, Gunma University
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