Incorporation of Testosterone and 5α-Dihydrotestosterone into Rat Ventral Prostate <I>in vitro</I>
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概要
- 論文の詳細を見る
Incubations of rat ventral prostate with testosterone-14C and 5a-dihydrotestosterone (5α-androstane-17β-ol-3-one)-<SUP>3</SUP>H revealed the gradual increase in uptake of both steroids into the tissue. Ratios of <SUP>3</SUP>H/<SUP>14</SUP>C of the incorporated steroids were similar during the incubation periods, and this indicated that incorporation of both the steroids proceeded in the same pattern. Radioactivity in purified 5α-dihydrotestosterone from the incubated whole tissues showed a gradual increase in both <SUP>14</SUP>C and <SUP>3</SUP>H, and <SUP>3</SUP>H/<SUP>4</SUP>C ratio was also almost constant throughout the incubation period. Therefore, rate of the conversion of testosterone to 5α-dihydrotestosterone seems to be constant during the incubation.<BR>A considerable difference in <SUP>3</SUP>H/<SUP>14</SUP>C ratios of ethyl acetate extracts was observed between the various subcellular components after 30 min incubation. <SUP>3</SUP>H/<SUP>14</SUP>C ratio in 5α-dihydrotestosterone, however, showed a small difference in all subcellular components. Most of the radioactivity incorporated into purified nuclei was attached to 5adihydrotestosterone.
- 社団法人 日本内分泌学会の論文
著者
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Shimazaki Jun
Department Of Ophthalmology Tokyo Dental College
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SATO JIN
Department of Urology, Gunma University School of Medicine
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Nagai Hisako
Department Of Biology Faculty Of Sciences Kyushu University
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SHIDA KEIZO
Department of Urology, Gunma University School of Medicine
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Shimazaki Jun
Department Of Ophthalmology School Of Medicine Keio University
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Sato Jin
Department Of Urology Gunma Cancer Center
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SHIMAZAKI JUN
Department of Dermatology, School of Medicine, Chiba University
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SHIDA KEIZO
Department of Urology School of Medicine, Gunma University
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SHIMAZAKI JUN
Department of Urology, Gunma University School of Medicine
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SATO JIN
Department of Clinical Pathology and Metabolism, Dokkyo University School of Medicine
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NAGAI HISAKO
Department of Urology, Gunma University School of Medicine
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