チアミンによる銅の新けい光定量法

概要

論文の詳細を見る
The previous paper had shown that the red-fluorescent product was formed by reaction of thiolized-thiamine with cuprous ion, and it could be applied to the analysis of thiamine in spot test and extracting method. In this paper, a new method for the fluorometric determination of copper with thiamine has been presented. The thiolized-thiamine reagent was prepared by dissolving thiamine hydrochloride in sodium hydroxide solution at pH 11.011.5. The sample solution was prepared by reducing cupric ion to cuprous ion with hydroxylamine sulfate, and adjusting pH to 7.07.2. The reaction product of cuprous-thiolized thiamine system was extracted with iso-amylalcohol involving 30 μg/ml butyl hydroxytoluene as a stabilizer, and the intensity of the red fluorescence at 650 mμ excited by 290 mμ rays was then measured. The limit of identification of copper in this method was 0.1 μg per ml. Further study showed that this red fluorescence was quite specific for copper ion, and no other cations and anions except sulfide, cyanide and phosphate quenched the fluorescence and interfered with the determination of copper.
社団法人日本分析化学会の論文