生体試料中の4-Nitroquinoline 1-Oxideおよびその代謝産物の定量法(第2報)4-Aminoquinolineの螢光定量法

概要

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Following the previous report on the determination of 4-nitroquinoline 1-oxide (4-NQO), which is a chemical carcinogen, and its metabolites, 4-hydroxyaminoquinoline 1-oxide (4-HAQO), 4-aminoquinoline 1-oxide (4-AQO), and 4-hydroxyquinoline 1-oxide (4-(OH)QO) in biological materials, fluorimetric determination of 4-aminoquinoline (4-AQ), which is the last reduced metabolite of 4-NQO, was established. After the development on a silica gel thin-layer chromatography, a single band of Rf 0.15,corresponding to 4-AQ, was extracted with HCl-MeOH (Rfs of 4-HAQO 0.60,4-AQO and 4-(OH)QO 0.45) and the fluorescence intensity was measured with excitation at 300 nm and emission at 355 nm. In addition a further modification of the determination of 4-NQO and its metabolites was attempted and a more rapid and more reliable determination was established.
公益社団法人日本薬学会の論文
1974-12-25