銅塩によるビタミンB_1の螢光分析法の研究 : (I)ビタミンB_1の定性分析法

概要

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The previous paper has shown that the red-fluorescent product was formed by reaction of thiolized thiamine with cupric ion, and it could be applied to the spot test on a filter paper of thiamine. In this paper, cuprous ion was compared with cupric ion in the above spot test. As a result, the sensitivity of the reaction by cuprous ion could be rised about 10 times, that is, 3μg/drop in the previous method (Cu^<2+>) but 0.25μg/drop in this method(Cu^+). Cuprous ion reagent was obtained by reduction of cupric ion with hydroxylamine and adjusted at pH 7.1 to 7.3. The reaction product from thiolized thiamine and cuprous ion was soluble in organic solvents, such as n-butanol or iso-amylalcohol, and showed the stable red flurescence. Therefore, the extraction method for the detection of thiamine was also examined. The limit of identification by this method was 5μg/ml. This reaction was interfered with Hg^<2+>, S-containing compound or riboflavin.
日本ビタミン学会の論文
1967-09-25