生体内抗酸化性成分存在下におけるチオバルビツール酸(TBA)反応

概要

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In our previous reports, it was assumed that the thiobarbituric acid (TBA) value not only gives the xisting amount of TBA reactive substances, but represents the combined status of tissue peroxidation including several antioxidative factors. For the further confirmation of the above assumption, biological antioxidants ; glutathione, ascorbic acid, or α-tocopherol, were added individually or combined into the reaction vessel of liver homogenate and methyl linoleate hydroperoxide (MLHPO) with TBA. The TBA value of MLHPO was depressed by the addition of glutathione but not influenced by ascorbic acid or α-tocopherol. The combined addition of these antioxidants showed an effective inhibition on the TBA reaction of MLHPO. The TBA reaction of control liver homogenate was strongly inhibited by ascorbic acid, but insignificantly influenced by α-tocopherol. The TBA reaction of CCl_4-intoxicated liver homogenate was significantly inhibited by α-tocopherol.
公益社団法人日本薬学会の論文
1983-08-25