光学活性移動相を用いた高速液体クロマトグラフィーによる製剤中のD-及びL-ドーパの定量(<特集>医薬品の分析)

概要

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The high performance liquid chromatography using chiral mobile phase containing L-phenylalanine-copper (II) complex was evaluated for purity test and stability test of L-Dopa preparations. The influences of the complex concentration and methanol content in the mobile phase on the separation of D- and L-Dopas were studied. The complex concentration had little influence on the separation, while the decrease in methanol content increased the retention time and the resolution. The detection limits obtained with UV detector and electrochemical detector were compared, and it was found that electrochemical detector could detect much less D-Dopa in the presence of L-Dopa than UV detector. D- and L-Dopas in tablets were determined simultaneously by connecting a UV detector and an electrochemical detector in series.
社団法人日本分析化学会の論文
1986-03-05