ケノデオキシコール酸とウルソデオキシコール酸の培養肝細胞に対する影響とPolyenphosphospatidyl cholinによる肝細胞保護作用について

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The cytotoxicity of CDCA and UDCA on primary cultured rat hepatocytes was investigated, and the cytoprotective effect of PPC on them was also studied.<BR>1mMI/L of CDCA in the cultured medium showed cytotoxicity on cultured hepatocytes incubated for 15 minutes, and presented marked cytotoxicity on them for 2 hours incubation.<BR>The electron microscopy revealed severe changes predominantly on the plasma membranes of cultured hepatocytes, and the light microscopy demonstrated decreased cell viability by trypan blue stain. The intracytoplasmic enzymes (GOT, GPT, LDH, OCT) were significantly elevated in the cultured medium.<BR>1mM/L of UDCA showed minimal cytotoxicity, compared with one of CDCA. 1mM/L of CDCA in the cultured medium containing PPC (1mg/ml) presented reduced cytotoxicity than CDCA (1mMIL) alone.<BR>These data indicated that 1mM/L of CDCA had the direct cytotoxicity on cultured hepatocytes while 1mM/L UDCA possessed slight cytotoxicity on them, and that PPC inhibited the cytotoxicity of CDCA on cultured hepatocytes.
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