Flow cytometric analysis of mononuclear cells infiltrating into liver in experimental autoimmune hepatitis in rats.

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The development of autoimmune hepatitis produced by immunizing Wistar rats with syngeneic liver proteins appears to be strain-specific since Lewis rats were relatively resistant, whereas Wistar rats are fully susceptible. Mononuclear cells infiltrating into liver (MIL) were indentified by immunohistochemical techniques with monoclonal antibodies (MoAb) specific for subpopulations of rat lymphocytes. In the portal areas there is a marked enrichment for RLyt-2 positive cytotoxic T lymphocytes (CTL). Subpopulations of MIL, spleen cells and peripheral blood lymphocytes (PBL) were indentified by immunofluorescence flow cytometry. We found the relatively small number of RLyt-1 positive cells as compared to the sum of RLyt-2 positive cells and W3/25 positive cells in MIL and PBL. Reactivity of various MoAb revealed that a population of RLyt-2 positive, but RLyt-1 negative or dull positive T lymphocytese is present in the liver. These findings support the notion that liver cell injury in experimental autoimmune hepatitis may, at least in part, be mediated by CTL.
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