血漿主要遊離アミノ酸の迅速簡易定量法とその臨床的応用とくに定量法の開発と各種肝疾患における鑑別診断への応用について

概要

論文の詳細を見る
To establish early diagnosis and accurate prognosis for acute liver failure, the author developed a new rapid and simplified assay method of plasma major free neutral amino acids: valine, isoleu cine, leucine, tyrosine, phenylalanine and methionine.<BR>High performance liquid chromatography was carried out, using an ion exchange resin (Kyowa Seimitsu Co., 6221OF) and citric acid buffers, pH 4.00, pH 4.25. By this method one sample was assayed within 20 minutes.<BR>Discriminant analysis, carried out using six amino acids in various liver diseases, exhibited a complete (100%) correct classification between fulminant and acute hepatitis, and an absolute value of vector for methionine was found to be the greatest among 6 amino acids. Whereas that of vector for phenylalanine was the highest between presence and absence of encephalopathy in decom pensated liver cirrhosis. These results strongly suggest that methionine plays an important role in the development of hepatic coma in acute liver failure, whereas phenylalanine is the most responsible for encephalopathy due to chronic liver failure.
社団法人日本肝臓学会の論文