各種肝疾患における肝組織内Hepatitis B core抗原の検索とその臨床的意義

概要

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The indirect immunofluorescence technique was employed to detect HBc-Ag in liver tissue of various liver diseases. The liver tissues to be tested were incubated with the primary sera with anti-HBc, but with no anti-HBs, and then were stained with FITC-labelled goat anti-human immunoglobulins.<BR>The specific fluorescence for HBc-Ag was observed in the nuclei or peri-nuclear area of the hepatocytes. Three patterns of the localization of HBc-Ag in hepatocyte were observed, that are; (1) intranuclear, diffuse, (2) intranuclear, speckled and (3) nucleomembranous or perinuclear cytoplasmic.<BR>HBc-Ag in hepatic tissue was positive in 13 cases out of 22 with various liver diseases positive for HBs-Ag in serum. They were 4 out of 12 asymptomatic HBs-Ag carriers, 6 out of 7 chronic hepatitis and each one of cirrhosis, primary hepatoma and malignant reticulosis with hepatic fibrosis, respectively. No HBc-Ag was detected in the liver of 9 patients with negative HBs-Ag in serum.
社団法人日本肝臓学会の論文