Studies on human peripherial blood B-lymphocyte colony formation in vitro

概要

論文の詳細を見る
A method was described about normal human peripherial B-lymphocyte colony formation in vitro. Irradiated T lymphocytes, and non-T cell population were cultured with 30 μg of phytohaemagglutinin-p (PHA-P) into methylcellulose gel, after a day of preincubation with PHA-P, and these lymphocytes. Colonies more than 50 cells were counted under a dissecting microscope.As a result, a linear relationship between the number of seeded cells and the growth of colonies was seen in methylcellulose gel. 570±333 colonies per 5.0×105 seeded cells were detected at 4 days of culture. Cells in colonies had surface immunoglobulin by using FITC-labeled anti-human immunoglobulin F (ab)2 rabbit serum under a fluolescent microscope.And cells in colonies had characters differentiated to plasma cells and lymphocytes in Giemsa stain, but no E rosette-forming ability.
日本臨床免疫学会の論文