Effects of Quercetin on Human α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Receptor-Mediated Ion Currents
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概要
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Quercetin is a low molecular weight flavonoid found in dietary fruits and vegetables. Quercetin, like other flavonoids, has demonstrated neuroprotective effects in vitro and in vivo. However, relatively little is known about how quercetin achieves its neuroprotective abilities. The α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor is one of several excitatory receptors, which play an important role in postsynaptic neurotransmission. Over-stimulation of ionotropic glutamate receptor including AMPA receptors is closely associated with excitatory neurotoxicities. In the present study, we investigated the effects of quercetin on the glutamate-induced inward current (IGlu) in Xenopus oocytes that heterologously express human AMPA receptor and stargazin, an auxiliary subunit of AMPA receptor. IGlu was measured using the two-electrode voltage clamp technique. In oocytes injected with cRNAs coding AMPA receptor (GluR1) and stargazin, quercetin inhibited IGlu in a reversible and concentration-dependent manner. The IC50 was 84.9±15.0 μM. Quercetin action on IGlu was attenuated by increasing glutamate concentration, and was membrane holding potential-dependent. These results show a possibility that quercetin interacts with AMPA receptor, which was heterologously expressed in Xenopus oocytes and that quercetin action on IGlu of AMPA receptor could be one of contributions of quercetin-mediated neuroprotections.
- 公益社団法人 日本薬学会の論文
著者
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KIM Hyoung-Chun
Neuropsychopharmacology and Toxicology Program, College of Pharmacy, Kangwon National University
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Lee Jun-ho
Department Of Physiology College Of Oriental Medicine Kyung-hee University
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RHIM Hyewhon
Life Science Division, KIST
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LEE Byung-Hwan
Department of Physiology, College of Veterinary Medicine, Konkuk University
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PYO Mi
Department of Physiology, College of Veterinary Medicine, Konkuk University
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CHOI Sun-Hye
Department of Physiology, College of Veterinary Medicine, Konkuk University
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SHIN Tae-Joon
Department of Physiology, College of Veterinary Medicine, Konkuk University
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LEE Sang-Mok
Department of Physiology, College of Veterinary Medicine, Konkuk University
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NAH Seung-Yeol
Department of Physiology, College of Veterinary Medicine, Konkuk University
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Choi Sun
Ginsentology Research Laboratory And Department Of Physiology College Of Veterinary Medicine Konkuk
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Nah Seung-yeol
Department Of Physiology College Of Veterinary Medicine Chonnam National University
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Lee Byung-hwan
Ginsentology Research Laboratory And Department Of Physiology College Of Veterinary Medicine Konkuk
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Park Ji-ho
Graduate School Of East-west Medical Science Kyung Hee University
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Hwang Sung-Hee
Department of Physiology, College of Veterinary Medicine, Konkuk University
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Kim Bo-Ra
Department of Physiology, College of Veterinary Medicine, Konkuk University
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Kim Hyoung-chun
Neuropsychopharmacology And Toxicology Program Coll. Of Pharmacy Kangwon National Univ.
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Han Ye
Advanced Technol. Fusion Konkuk Univ.
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Rhim Hyewhon
Life Sci. Div. Korea Inst. Of Sci. And Technol.
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Park Ji-ho
Graduate School Of East-west Medical Sci. Kyung Hee Univ.
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Pyo Mi
Department Of Physiology College Of Veterinary Medicine Konkuk University
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Shin Tae-joon
Dep. Of Physiology Coll. Of Veterinary Medicine And Bio/molecular Informatics Center Konkuk Univ.
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Hwang Sung-Hee
Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University
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PARK Ji-Ho
Graduate School of East-West Medical Science, Kyung Hee University
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Han Ye
Advanced Technology Fusion, Konkuk University
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Lee Byung-Hwan
Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University
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Hwang Sung-Hee
Department of Applied Physics, Hanyang University, Ansan, Gyeonggi-do 426-791, Korea
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Nah Seung-Yeol
Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University
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Rhim Hyewhon
Life Science Division, Korea Institute of Science and Technology
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Shin Tae-Joon
Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University
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Choi Sun-Hye
Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University
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Pyo Mi
Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University
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Lee Sang-Mok
Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University
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Lee Jun-Ho
Department of Physiology, College of Oriental Medicine, Kyung Hee University
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Kim Bo-Ra
Department of Physiology, College of Veterinary Medicine and Bio/Molecular Informatics Center, Konkuk University
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