Determination of Ginsenoside Rf and Rg_2 from Panax ginseng Using Enzyme Immunoassay

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概要

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• We have developed an enzyme immunoassay (EIA) to quantify trace amounts of ginsenoside Rf (Rf), one of the glycosides of protopanaxatriol from Panax ginseng. A carrier protein of bovine serum albumin (BSA) was coupled to the carbohydrate component of Rf using the periodate oxidation method. Antibodies were raised in rabbits using Rf-BSA conjugate as the immunogen and competitive indirect EIA was used for the determination of Rf. The working range was 0.01-10 ng per assay. The anti-Rf antiserum cross-reacted with ginsenoside Rg_2 (105%), which is also a component of Panax ginseng and has a very similar chemical structure to Rf. These results suggest that the anti-Rf antiserum could also be used for the quantitation of ginsenoside Rg_2 as well as ginsenoside Rf. In a comparison of EIA and HPLC the linear regression equation and correlation coefficient for the two methods were y(EIA)=1.31x (HPLC)-11.48 and 0.98,respectively.

• 公益社団法人日本薬学会の論文
• 1998-07-15

著者

• NAH Seung-Yeol

  Department of Physiology, College of Veterinary Medicine, Konkuk University

• KIM Si-kwan

  Korea Ginseg & Tobacco Research Institute

• Kim S‐c

  Korea Ginseng And Tobacco Research Institute

• Nah Seung-yeol

  Ginsentology Research Laboratory And Department Of Physiology College Of Veterinary Medicine Konkuk

• Nah Seung-yeol

  Department Of Physiology College Of Veterinary Medicine Chonnam National University

• Nah Seung-yeol

  Bio Molecular Informatics Center Konkuk University

• Nah Jin-ju

  Department Of Physiology College Of Veterinary Medicine Chonnam National University

• Jung Da-woon

  Department Of Physiology College Of Veterinary Medicine Chonnam National University

• KIM Seok-Chang

  Korea Ginseng and Tobacco Research Institute

• YOON So-Rah

  Department of Physiology, College of Veterinary Medicine Chonnam National University

• NAM Ki-Yeul

  Korea Ginseng and Tobacco Research Institute

• Yoon So-rah

  Department Of Physiology College Of Veterinary Medicine Chonnam National University

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