Use of Formalin-Fixed Paraffin-Embedded Tissue and Single-Strand Conformation Polymorphism Analysis for Polymerase Chain Reaction of Antigen Receptor Rearrangements in Dogs

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概要

• 論文の詳細を見る

• PCR for antigen receptor gene rearrangement analysis (PARR) is a new diagnostic method for lymphoid neoplasia. In PARR using formalin-fixed paraffin-embedded tissues (PARR-FFPE), control DNA amplification was successful in only three of five samples. The formalin fixation times of the three samples were shorter than those of the others. Analysis of the formalin fixation time and DNA amplification controls suggested that a formalin fixation time of less than one week is appropriate. Additionally, application of single strand conformation polymorphism (SSCP) for PARR provided clearer results than conventional PARR in 16 unfixed tissues and three FFPE tissues. These results show that PARR-FFPE is viable for tissues with an appropriate formalin fixation time and that application of FFPE and SSCP for PARR are useful for diagnosis and retrospective study of canine lymphoid neoplasia.

• 社団法人　日本獣医学会の論文

著者

• SHIMOKAWA MIYAMA

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agricultur

• HIRAOKA Hiroko

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agricultur

• OKUDA Masaru

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agricultur

• MIZUNO Takuya

  Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agricultur

• HAYASHI Toshiharu

  Laboratory of Veterinary Pathology, Faculty of Agriculture, Yamaguchi University

• MORIMOTO Masahiro

  Laboratory of Veterinary Pathology, Faculty of Agriculture, Yamaguchi University

• Kaneko Naoki

  Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University

• ITAMOTO Kazuhito

  Laboratory of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University

• Tanimoto Takashi

  Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University

• UNE Satoshi

  Laboratory of Veterinary Clinical Diagnosis, Faculty of Agriculture, Yamaguchi University

• Hayashi Toshiharu

  Laboratory Of Veterinary Pathology Faculty Of Agriculture Yamaguchi University

• Mizuno Takuya

  Laboratories Of Veterinary Internal Medicine Dep. Of Veterinary Medicine Fac. Of Agriculture Yamaguchi Univ. ...

• OKUDA Masaru

  Laboratories of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan

• HIRAOKA Hiroko

  Laboratories of Veterinary Clinical Pathology, Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan

• ITAMOTO Kazuhito

  Laboratories of Veterinary Clinical Diagnostics, Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan

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