Molecular Cloning of the Canine Fragile Histidine Triad (FHIT) Gene and Fhit Protein Expression in Canine Peripheral Blood Mononuclear Cells
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概要
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A fragile histidine triad (FHIT) gene has been studied as a tumor-associated gene in humans. The aberrant FHIT gene and its protein expression have been reported in many types of human cancers. The present study explored the canine FHIT gene structure and its protein expression in the peripheral blood mononuclear cells of healthy dogs by RT-PCR, RACE and immunoblot analysis. The obtained canine FHIT gene contained nine small exons and was located on canine chromosome 20. Furthermore, we identified an alternative splicing form of the FHIT transcript. The deduced amino acid sequence was well conserved between species, and anti-human Fhit antibody could be used to detect the canine Fhit protein. These findings will be useful for future research.
- 社団法人 日本獣医学会の論文
著者
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SHIMOKAWA MIYAMA
Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agricultur
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HIRAOKA Hiroko
Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agricultur
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OKUDA Masaru
Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agricultur
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MIZUNO Takuya
Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agricultur
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Hiraoka Hiroko
Yamaguchi Univ. Yamaguchi Jpn
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Hiraoka Hiroko
山口大学 農学部獣医内科学
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Minami Koji
Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University
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Kaneko Naoki
Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University
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