Lack of Prolactin (PRL) mRNA Expression in Estrogen-treated Rat Mammary Glands
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概要
- 論文の詳細を見る
Mammary gland development and hyperprolactinemia of rats were induced by estradiol (E2) treatment. The condition of the animals resembled that of during lactation. When it focused on high PRL levels, we studied the molecular phenotype of the rat mammary gland following E2 treatment, targeting prolactin (PRL) mRNA, using the sensitive techniques of solution and in situ reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, we compared the PRL mRNA and plasma PRL level with those in the mammary glands of both pregnant and lactating rats. In the E2-treated groups, the plasma PRL levels were increased. No mammary PRL mRNA was detected in E2-treated rats by either solution or in situ RT-PCR. The plasma PRL level was extremely high and associated with the mammary gland development in lactating rats. In addition, mammary PRL mRNA was detected in both pregnancy and lactation by solution and in situ RT-PCR. These results suggested a lack of PRL mRNA in the mammary gland of E2-treated rats, despite similar plasma PRL levels and morphological changes to those in lactating rats. The induction of PRL mRNA coincident with the development of mammary gland in the pregnant and lactating rats, differed from that in the E2-treated rats in molecular biology, particularly in PRL mRNA expression, and suggested that PRL mRNA in mammary gland contributes to de novo synthesis of PRL in milk which is under the multihormonal regulation.
- 日本毒性病理学会の論文
- 1999-12-01
著者
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MIYAJIMA Katsuhiro
Toxicology Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco Inc.
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Kakimoto Kochi
Department of Pathology Tokai University School of Medicine
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Umemura Shinobu
Department of Pathology, Tokai University School of Medicine
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KOIZUMI Haruko
Toxicology Research Laboratories, Central Pharmaceutical Research Institute
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SHODA Toshiyuki
Toxicology Research Laboratories, Central Pharmaceutical Research Institute
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TAKAHASHI Akemi
Toxicology Research Laboratories, Central Pharmaceutical Research Institute
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MIYAKAWA Yoshifumi
Toxicology Research Laboratories, Central Pharmaceutical Research Institute
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Osamura R
Department Of Pathology School Of Medicine Tokai University
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Takahashi A
Toxicology Research Laboratories Central Pharmaceutical Research Institute
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Koizumi H
Toxicology Research Laboratories Central Pharmaceutical Research Institute
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Koizumi Haruko
日本たばこ産業
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Osamura Robert
Dep. Of Pathology Tokai Univ. School Of Medicine
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Takahashi Akemi
Toxicology Research Laboratories Central Pharmaceutical Research Institute
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IWASAKA Toshiki
Department of Pathology, Tokai University School of Medicine, Toxicology Research Laboratories, Cent
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Osamura R
Tokai Univ. School Of Medicine Kanagawa Jpn
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Osamura R.yoshiyuki
Department Of Pathology Tokai University School Of Medicine
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Osamura R.
Tokai Univ. School Of Medicine Kanagawa Jpn
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Umemura Shinobu
Department Of Pathology & Division Of Diagnostic Pathology Tokai University School Of Medicine
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Shoda Toshiyuki
Toxicology Research Laboratories Central Pharmaceutical Research Institute Japan Tobacco Inc.
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Shoda Toshiyuki
Division Of Pathology National Institute Of Health Sciences
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Hitoshi Koizumi
Toxicology Research Laboratories Central Pharmaceutical Research Institute
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Iwasaka Toshiki
Toxicology Research Laboratories Central Pharmaceutical Research Institute
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Koizumi Haruko
Toxicology Research Laboratories Central Pharmaceutical Research Institute Japan Tobacco Inc.
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Miyajima Katsuhiro
Toxicology Res. Laboratories Central Pharmaceutical Res. Inst. Japan Tobacco Inc.
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Osamura R
Tokai Univ. School Of Medicine
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Iwasaka Toshiki
Department Of Pathology Tokai University School Of Medicine
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Miyakawa Yoshifumi
Toxicology Research Laboratories Central Pharmaceutical Research Institute Japan Tobacco Inc.
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Miyajima Katsuhiro
Toxicology Research Laboratories Central Pharmaceutical Research Institute Japan Tobacco Inc.
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Miyajima Katsuhiro
Japan Tobacco Inc. Central Pharmaceutical Research Institute Toxicology Research Laboratories
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Umemura S
Tokai Univ. School Of Medicine
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Shoda Toshiyuki
Toxicology Research Lab. Central Pharmaceutical Research Institute Japan Tobacco Inc.
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Takahashi Akemi
Toxicology Research Lab. Central Pharmaceutical Research Institute Japan Tobacco Inc.
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