Intermediate structure between chromatin fibers and chromosome revealed by mechanical stretching and SPM measurement
スポンサーリンク
概要
- 論文の詳細を見る
The morphology of chromosomes (certain rod-shaped structures) is highly reproducible despite the high condensation of chromatin fibers (∼1 mm) into chromosomes (∼1 μm). However, the mechanism underlying the condensation of chromatin fibers into chromosomes is unclear. We assume that investigation of the internal structure of chromosomes will aid in elucidating the condensation process. In order to observe the detailed structure of a chromosome, we stretched a human chromosome by using a micromanipulator and observed its morphology along the stretched region by scanning probe microscopy (SPM). We found that the chromosome consisted of some fibers that were thicker than chromatin fibers. The found fiber was composed of approximately 90-nm-wide beads that were linked linearly. To explore the components of the fiber, we performed immunofluorescence staining of the stretched chromosome. Fluorescence signals of topoisomerase (Topo) IIα, which is known to interact with and support chromatin fibers, and DNA were detected both on the found fiber and beads. Furthermore, after micrococcal nuclease and trypsin treatments, the fibers were found to be mechanically supported by proteins. These results suggest that chromosome comprises an intermediate structure between chromatin fibers and chromosomes.
- 2010-09-10
著者
-
Kawabata K
Division Of Physics Graduate School Of Science Hokkaido University
-
Haga H
Div. Of Biological Sciences Graduate School Of Sci. Hokkaido Univ.
-
Haga Hisashi
北海道大学 生物化学科
-
Haga Hisashi
Department Of Physics Faculty Of Science Hokkaido University
-
Ushiki T
Niigata Univ. Graduate School Of Medical And Dental Sci. Niigata
-
Ushiki Tatsuo
Division Of Microscopic Anatomy And Bio-imaging Department Of Cellular Function Niigata University G
-
Ushiki Tatsuo
Division Of Microscopic Anatomy And Bioimaging Graduate School Of Medical And Dental Sciences Niigat
-
Mizutani Takeomi
Transdisciplinary Life Science Course Faculty Of Advanced Life Science Hokkaido University
-
Ikeda Kensuke
Divisions Of Biological Sciences Graduate School Of Science Hokkaido University
-
KAWABATA KAZUSHIGE
Transdisciplinary, Life Science Course, Faculty of Advanced Life Science, Hokkaido University
-
Haga Hisashi
Division Of Biological Sciences Graduate School Of Science Hokkaido University
-
Kawabata K
Transdisciplinary Life Science Course Faculty Of Advanced Life Science Hokkaido University
-
Kawabata Kazushige
Division Of Biological Sciences Graduate School Of Science
関連論文
- Integrin β1-dependent invasive migration of irradiation-tolerant human lung adenocarcinoma cells in 3D collagen matrix
- Intermediate structure between chromatin fibers and chromosome revealed by mechanical stretching and SPM measurement
- Mechanical Properties of Membrane Surface of Cultured Astrocyte Revealed by Atomic Force Microscopy
- Time-lapse viscoelastic imaging of living fibroblasts using force modulation mode in AFM
- Quantitative analyses of topography and elasticity of living and fixed astrocytes
- Dynamics of Astrocyte Adhesion as Analyzed by a Combination of Atomic Force Microscopy and Immunocytochemistry : the Involvement of Actin Filaments and Connexin 43 in the Early Stage of Adhesion
- Reexamination of Fine Surface Topography of Nerve Cells Revealed by Atomic Force Microscopy
- Atomic Force Microscopic Observation of Three-Dimensional Morphological Changes of Neurons When Stimulated by a Neurotransmitter
- Surface Structures of Cultured Type 2 Astrocytes Revealed by Atomic Force Microscopy(Neurobiology)
- Imaging Elastic Properties of Soft Materials Immersed in Water Using Force Modulation Mode irn Atomic Force Mieroscopy