Mechanical Response to Isotropic Shrinkage of Fibroblasts Measured by Scanning Probe Microscopy
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概要
- 論文の詳細を見る
Mechanical stimuli such as cyclic stretch and fluid stress affect various cellular physiologies, including proliferation, morphology, and differentiation. We investigated cellular response to shrinking stimuli by developing an isotropic deformation device and observing cellular elasticity with mechanical scanning probe microscopy (M-SPM). The isotropic deformation device consists of a steel ring and a deformable elastic culture dish made of transparent silicone rubber. The M-SPM can visualize topography and spatial distribution of local elasticity of biomaterials in solution. Fibroblasts became softer in response to 6% shrinkage. Cell elasticity did not increase for 1 h after the shrinking stimulus. Inhibitory studies using lysophosphatidic acid and calyculin-A revealed that myosin light chain phosphatase leading to dephospholylation of myosin II regulatory light chain is involved in cell softening.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2008-07-25
著者
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Haga Hisashi
Department Of Physics Faculty Of Science Hokkaido University
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Tamura Kazushi
Department Of Pediatrics And Developmental Medicine Gunma University Graduate School
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Kawabata Kazushige
Department of Biological Sciences, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan
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Kawamoto Taisuke
Department of Biological Sciences, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan
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Mizutani Takeomi
Department of Biological Sciences, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan
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Tamura Kazushi
Department of Biological Sciences, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan
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Haga Hisashi
Department of Biological Sciences, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan
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