Visualization of Stretch-Induced Intracellular Tensional Response of Single Fibroblasts by Mechanical Scanning Probe Microscopy
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概要
- 論文の詳細を見る
This study was aimed at developing a novel experimental setup to investigate the tensional response of a single living cell to external deformation. We constructed the setup which consisted of three components: a device for applying various patterns of uniaxial deformation to living cells, a mechanical scanning probe microscope (M-SPM), and an optical microscope. Intracellular tension is reflected by cellular stiffness measured by M-SPM. Using the setup, we found that stiffness in some areas on a living fibroblast cell increased while stiffness in other areas decreased under 8% external single-step stretch. Furthermore, we found that repetitive deformation inhibited the increase in the stretch-induced cellular stiffness. The present setup is useful for investigating the characteristics of intracellular tensional responses to external deformation.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2007-08-30
著者
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MIZUTANI Takeomi
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Tamura Kazushi
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Kawabata Kazushige
Division Of Biological Sciences Graduate School Of Science
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Haga Hisashi
Division Of Biological Sciences Graduate School Of Science
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Tamura Kazushi
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan
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