Observation of Stiff Domain Structure on Collagen Gels by Wide-Range Scanning Probe Microscopy
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概要
- 論文の詳細を見る
Spatial distributions for 100-μm2-stiffness of type I collagen gels were measured by wide-range scanning probe microscopy. A cantilever being attached to the tip of a glass bead of 100 μm diameter was used to reduce local stress during measurements. This method enabled the cantilever to be in contact with the gel surface in a manner of a Hertzian contact model regardless of the rough meshwork formation of collagen gels. Stiffness images of collagen gels showed stiff domain structures with a size of 20 μm. The more the concentration of collagen increased, the more the stiffness increased with the growth of stiff domain structures. Immunostaining for collagen molecules showed highly concentrated fibril structures as large as the stiff domain structures. These results indicate that the structure of collagen gel is nonuniform in the range of 100 μm$\square$, but it is heterogeneous because of collagen fibril aggregation.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2006-03-15
著者
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MIZUTANI Takeomi
Division of Biological Sciences, Graduate School of Science, Hokkaido University
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Kato Kosaku
Division Of Biological Sciences Graduate School Of Science Hokkaido University
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Kawabata Kazushige
Division Of Biological Sciences Graduate School Of Science
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Haga Hisashi
Division Of Biological Sciences Graduate School Of Science
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Kawabata Kazushige
Division of Biological Sciences, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan
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Matsuda Kenta
Division of Biological Sciences, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan
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Mizutani Takeomi
Division of Biological Sciences, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan
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Haga Hisashi
Division of Biological Sciences, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan
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