Contribution of cellular contractility to spatial and temporal variations in cellular stiffness
スポンサーリンク
概要
- 論文の詳細を見る
Scanning probe microscopy and immunofluorescence observations indicated that cellular stiffness was attributed to a contractile network structure consisting of stress fibers. We measured temporal variations in cellular stiffness when cellular contractility was regulated by dosing with lysophosphatidic acid or Y-27632. This experiment reveals a clear relation between cellular stiffness and contractility: Increases in contractility cause cells to stiffen. On the other hand, decreases in contractility reduce cellular stiffness. In both cases, not only the stiffness of the stress fibers but also that of the whole of the cell varies. Immunofluorescence observations of myosin II and vinculin indicated that the stiffness variations induced by the regulation of cellular contractility were mainly due to rearrangements of the contractile actin network on the dorsal surface. Taken together, our findings provide evidence that the actin cytoskeletal network and its contractility features provide and modulate the mechanical stability of adherent cells.
- Elsevier Inc.の論文
- 2004-11-01
著者
-
Kawabata K
Division Of Physics Graduate School Of Science Hokkaido University
-
Haga H
Div. Of Biological Sciences Graduate School Of Sci. Hokkaido Univ.
-
Haga Hisashi
北海道大学 生物化学科
-
Haga Hisashi
Department Of Physics Faculty Of Science Hokkaido University
-
KAWABATA KAZUSHIGE
Transdisciplinary, Life Science Course, Faculty of Advanced Life Science, Hokkaido University
-
Haga Hisashi
Division Of Biological Sciences Graduate School Of Science Hokkaido University
-
Kawabata K
Transdisciplinary Life Science Course Faculty Of Advanced Life Science Hokkaido University
-
Kawabata Kazushige
Division Of Biological Sciences Graduate School Of Science
関連論文
- Integrin β1-dependent invasive migration of irradiation-tolerant human lung adenocarcinoma cells in 3D collagen matrix
- Intermediate structure between chromatin fibers and chromosome revealed by mechanical stretching and SPM measurement
- Mechanical Properties of Membrane Surface of Cultured Astrocyte Revealed by Atomic Force Microscopy
- Time-lapse viscoelastic imaging of living fibroblasts using force modulation mode in AFM
- Quantitative analyses of topography and elasticity of living and fixed astrocytes
- Dynamics of Astrocyte Adhesion as Analyzed by a Combination of Atomic Force Microscopy and Immunocytochemistry : the Involvement of Actin Filaments and Connexin 43 in the Early Stage of Adhesion
- Reexamination of Fine Surface Topography of Nerve Cells Revealed by Atomic Force Microscopy
- Atomic Force Microscopic Observation of Three-Dimensional Morphological Changes of Neurons When Stimulated by a Neurotransmitter
- Surface Structures of Cultured Type 2 Astrocytes Revealed by Atomic Force Microscopy(Neurobiology)
- Imaging Elastic Properties of Soft Materials Immersed in Water Using Force Modulation Mode irn Atomic Force Mieroscopy
- Comparative Atomic Force and Scanning Electron Microscopy for Fine Structural Images of Nerve Cells
- Fine Surface Images That Reflect Cytoskeletal Structures in Cultured Glial Cells by Atomic Force Mieroseopy
- Elastic Properties of Living Fibroblasts as Imaged Using Force Modulation Mode in Atomic Force Microscopy
- 2P-250 走査型プローブ顕微鏡による生きた海馬神経細胞シナプスの3次元形態観察(神経・感覚,第46回日本生物物理学会年会)
- Visualization of Elasticity Distribution of Single Human Chromosomes by Scanning Probe Microscopy
- 1P130 染色体の牽引時に生じる力の振動の観察(核酸-構造・物性,第48回日本生物物理学会年会)
- Preasymptotic 3D XY Critical Behavior of Heat Capacity at Normal-Incommensurate Phase Transition in K_2ZnCl_4 and K_2SeO_4
- Three Dimensional XY Critical Behavior of Heat Capacity at Normal-Incommensurate Phase Transition in Rb_2ZnCl_4
- New Phase Transition in Ferroelectric (CH_3NHCH_2COOH)_3CaCl_2 at Low Temperatures
- Critical Behaviour of Specific Heat in Ferroelectric K_2ZnCl_4 and (NH_4)_2BeF_4 at Normal-Incommensurate Phase Transition
- 3P-160 伸展刺激に対する細胞の硬化応答は細胞が2サイクル以上の周期伸展を記憶することで消失する(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
- Practical Scan Speed in Atomic Force Microscopy for Live Neurons in a Physiological Solution ( Scanning Tunneling Microscopy)
- AFM Observation of Three-Dimensional Fine Structural Changes in Living Neurons
- Diffusion Barrier Effect of Ultra-Thin Photo-Nitrided a-Si:H Overlayer on SnO_2/Glass Substrate
- Impurity Diffusion Barrier Effect of Ultra-Thin Plasma-Nitrided a-Si:H Overlayer on SnO_2/Glass Substrate
- Diffusion of constituent Atoms in P-type a-Si:H / SnO_2 Interfaces : Surfaces, Interfaces and Films
- 2P218 デジタル画像相関法を用いた生きた細胞における細胞骨格の動態解析(細胞生物的課題(接着,運動,骨格,伝達,膜),第48回日本生物物理学会年会)
- Nanoparticles with Multiple Perfluorocarbons for Controllable Ultrasonically Induced Phase Shifting
- 3P-159 デジタル画像相開法により得られた局所伸展刺激下における細胞骨格ネットワークの不均一な変形分布(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
- Static Friction of Agar Gels : Formation of Contact Junctions at Frictional Interface(Electromagnetism, Optics, Acoustics, Heat Transfer, Classical Mechanics and Fluid Mechanics)
- Microdomain structure of agar gels observed by mechanical-scanning probe microscopy
- Thermal and Dielectric Properties of a New Ferroelectric LaBGeO_5
- Neuronal components of the superior and inferior tentacles in the terrestrial slug, Limax marginatus
- Laser-Induced Oxygen Control in Small Region of YBa_2Cu_3O_y Poly- and Single Crystals, Observed by Microscopic IR Reflection
- Microscopic IR-Reflection Study of Oxygen Doping Effect Induced by Laser Irradiation on YBa_2Cu_3O_y, Surface
- Direct Observation of ^O Tracer Diffusion in a YBa_2Cu_3O_y Single Crystal by Secondary Ion Mass Spectrometry
- Matrixmetalloproteinases : up-regulated in subclones that survived 10-Gy irradiation
- 3P-158 リーディングラメラにおける粒状機械構造のアクチン細胞骨格依存的なダイナミクス : 高速生細胞SPMを用いた研究(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
- 3P-155 印加する外力の周波数に依存した細胞の生理的応答(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
- 3P-167 ストレスファイバー形成過程において出現するアクチン線維凝集体 : アクチン線維ネットワーク再編成に対してアクチン線維凝集体が果たし得る役割(細胞生物学的課題(3),第46回日本生物物理学会年会)
- Development of a device to stretch tissue-like materials and to measure their mechanical properties by scanning probe microscopy.
- Diphosphorylation of the myosin regulatory light chain enhances the tension acting on stress fibers in fibroblasts.
- 2P529 Tensional Responses of Single Living Cells to External Stretch Visualized by SPM(52. Bio-imaging,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P176 New filamentous ordered structure appeared by stretching of unfixed human chromosome(36. DNA to chromatin,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Spatiotemporal Variation in Cellular Stiffness Corresponding to Development of Morphological Polarity
- Contribution of cellular contractility to spatial and temporal variations in cellular stiffness
- Wide-Range Scanning Probe Microscopy for Visualizing Biomaterials in the Submillimeter Range
- Improvement of Force Modulation Mode with Scanning Probe Microscopy for Imaging Viscoelasticity of Living Cells
- Drastic change of local stiffness distribution correlating to cell migration in living fibroblasts.
- Visualization of stretch-induced intracellular tensional response of single fibroblasts by mechanical scanning probe microscopy (Special issue: Scanning probe microscopy)
- Wide range scanning probe microscopy for probing mechanical effects on cellular function
- Mechanical response of single myoblasts to various stretching patterns visualized by scanning probe microscopy
- Mechanical Response to Isotropic Shrinkage of Fibroblasts Measured by Scanning Probe Microscopy
- Observation of Stiff Domain Structure on Collagen Gels by Wide-Range Scanning Probe Microscopy
- Spatiotemporal Variation in Cellular Stiffness Corresponding to Development of Morphological Polarity
- The Role of Actin-Binding Protein Filamin A in Cellular Stiffness and Morphology Studied by Wide-Range Scanning Probe Microscopy
- Visualization of Stretch-Induced Intracellular Tensional Response of Single Fibroblasts by Mechanical Scanning Probe Microscopy
- Comparative Atomic Force and Scanning Electron Microscopy for Fine Structural Images of Nerve Cells