Wide-Range Scanning Probe Microscopy for Visualizing Biomaterials in the Submillimeter Range

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概要

• 論文の詳細を見る

• We developed wide-range scanning probe microscope (WR-SPM) for visualizing the topography and mechanical properties of samples in the submillimeter range. A piezoelectric scanner with a maximum $xy$ scan range of 400 μm square and a $z$ range of 16 μm was constructed by joining two commercial piezotranslators in tandem. The reliability of measurement was confirmed by measuring a well-defined pattern engraved on a silicon substrate. Using the WR-SPM, the spatial distribution of stiffness and time-lapse images of a large colony consisting of approximately 40 epithelial cells were visualized successfully, where the local stiffness was measured using the force mapping mode. These results indicate that the WR-SPM can be a powerful instrument to visualize the mechanical properties of biological phenomena extending in the submillimeter range.

• Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
• 2004-07-15

著者

• MIZUTANI Takeomi

  Division of Biological Sciences, Graduate School of Science, Hokkaido University

• Nemoto Koji

  Division Of Physics Graduate School Of Science Hokkaido University

• HAGA Hisashi

  Biological Sciences, Graduate School of Science, Hokkaido University

• KAWABATA Kazushige

  Biological Sciences, Graduate School of Science, Hokkaido University

• Kawabata Kazushige

  Biological Sciences, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan

• Mizutani Takeomi

  Divisions of Physics, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan

• Haga Hisashi

  Biological Sciences, Graduate School of Science, Hokkaido University, North 10 West 8, Kita-ku, Sapporo 060-0810, Japan

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